Gotzos V, Wintergerst E S, Musy J P, Spichtin H P, Genton C Y
Institute of Histology and General Embryology, University of Fribourg, Switzerland.
Am J Surg Pathol. 1999 Jun;23(6):701-11. doi: 10.1097/00000478-199906000-00010.
The expression of calretinin, a calcium-binding protein, has been studied in a series of 82 human colorectal adenocarcinomas. In 22.5% of the cases, part of the tumor cells were calretinin-positive, whereas the cells of the normal and paratumoral mucosa were always negative. Two types of cells from the tumoral mass reacted positively and selectively with calretinin-antisera: the tumor cells and giant fibroblasts. The neurons of enteric ganglia and reactive mesothelial cells also reacted positively to the same antibody. The results obtained by immunochemistry have been confirmed by Western blot analysis and in situ hybridization for calretinin mRNA. There is a correlation between the expression of calretinin and the degree of differentiation of the tumor. Well-differentiated tumors express calretinin in only 5% of the cases, whereas this percentage is 20% for moderately differentiated tumors and 66.6% for poorly differentiated or undifferentiated tumors. We conclude that calretinin is expressed by most undifferentiated colorectal adenocarcinomas, but only by a limited number of cells in well-differentiated tumors. The degree of its expression coincides also with additional signs of malignancy, such as an increase in the number of metastases in the regional lymph nodes and in other organs.
钙结合蛋白钙视网膜蛋白（calretinin）的表达情况已在82例人类结肠直肠癌中进行了研究。在22.5%的病例中，部分肿瘤细胞呈钙视网膜蛋白阳性，而正常和肿瘤旁黏膜细胞始终为阴性。肿瘤块中的两种细胞对钙视网膜蛋白抗血清呈阳性且选择性反应：肿瘤细胞和巨大成纤维细胞。肠神经节的神经元和反应性间皮细胞对同一抗体也呈阳性反应。免疫化学获得的结果已通过蛋白质印迹分析和钙视网膜蛋白mRNA的原位杂交得到证实。钙视网膜蛋白的表达与肿瘤的分化程度之间存在相关性。高分化肿瘤仅在5%的病例中表达钙视网膜蛋白，而中分化肿瘤的这一比例为20%，低分化或未分化肿瘤为66.6%。我们得出结论，大多数未分化的结肠直肠癌表达钙视网膜蛋白，但高分化肿瘤中只有有限数量的细胞表达。其表达程度也与其他恶性征象相符，如区域淋巴结和其他器官中转移灶数量的增加。
