蓝藻念珠藻6720中编码固氮酶还原酶（nifH）基因的克隆与核苷酸序列分析

Cloning and nucleotide sequence of the gene encoding dinitrogenase reductase (nifH) from the cyanobacterium Nostoc 6720.

作者信息

Beesley C E, Smith R J, Temple S J, Lea P J

机构信息

Department of Biological Sciences, I.E.B.S., Lancaster University, UK.

出版信息

Biochim Biophys Acta. 1994 Oct 18;1219(2):548-50. doi: 10.1016/0167-4781(94)90086-8.

DOI:10.1016/0167-4781(94)90086-8

PMID:7918657

Abstract

The nucleotide sequence of the 3' end of the nifU coding sequence, the complete coding sequence of nifH and a substantial part of the 5' end of nifD coding sequence from Nostoc 6720 is presented. The coding sequences are highly conserved with those of Anabaena 7120 and Anabaena sp. L31. However the intergenic region between nifU and nifH contains two segments of short tandemly repetitive repeat sequences (STRRs) that differ from the STRR that is common to both Anabaena7120 and Anabaenasp. L31. Various sequence structures that are common to Nostoc 6720, the Anabaena strains and Plectonema boryanum are discussed.

摘要

本文给出了念珠藻6720中nifU编码序列3'端的核苷酸序列、nifH的完整编码序列以及nifD编码序列5'端的大部分序列。这些编码序列与鱼腥藻7120和鱼腥藻L31的编码序列高度保守。然而，nifU和nifH之间的基因间隔区包含两段短串联重复序列（STRR），这与鱼腥藻7120和鱼腥藻L31共有的STRR不同。文中还讨论了念珠藻6720、鱼腥藻菌株和博氏织线藻共有的各种序列结构。