从普通念珠藻UTEX 584中克隆nifHD以及与棕色固氮菌nifU基因部分序列同源的侧翼区域。
Cloning of nifHD from Nostoc commune UTEX 584 and of a flanking region homologous to part of the Azotobacter vinelandii nifU gene.
作者信息
Defrancesco N, Potts M
机构信息
Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061.
出版信息
J Bacteriol. 1988 Jul;170(7):3297-300. doi: 10.1128/jb.170.7.3297-3300.1988.
Abstract
The heterocystous cyanobacterium Nostoc commune UTEX 584 contains two nifH-like sequences (nifH1 and nifH2) in addition to nifHD. A region of DNA 1 kilobase upstream from the 5' end of nifH showed considerable sequence similarity to part of the published nifU sequences of Azotobacter vinelandii and Klebsiella pneumoniae.
摘要
异形胞蓝细菌普通念珠藻UTEX 584除了含有nifHD外，还含有两个类nifH序列（nifH1和nifH2）。nifH 5'端上游1千碱基的一段DNA区域与已发表的棕色固氮菌和肺炎克雷伯菌的nifU序列的一部分显示出相当大的序列相似性。
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