Kessler P, Kotzyba-Hibert F, Leonetti M, Bouet F, Ringler P, Brisson A, Ménez A, Goeldner M P, Hirth C
Départment d'Ingéniérie et d'Etudes des Protéines (DIEP), CEA, C.E. Saclay, Gif-sur-Yvette, France.
Bioconjug Chem. 1994 May-Jun;5(3):199-204. doi: 10.1021/bc00027a004.
The regioselective modification of a snake curaremimetic toxin is described. Toxin-alpha from Naja nigricollis was derivatized with an electron-dense maleimido undecagold cluster 5. The cluster-bound obtained toxin 8 has a very high affinity for the cholinergic binding site (Ki = 70 pM) of Torpedo marmorata nicotinic receptor. It is harboring a compact heavy atom core of about 8 A which makes it very useful for electron microscopy experiments.
描述了一种蛇毒类箭毒毒素的区域选择性修饰。黑颈眼镜蛇的毒素α用电子致密的马来酰亚胺十一金簇5进行衍生化。得到的簇结合毒素8对电鳐烟碱受体的胆碱能结合位点具有非常高的亲和力（Ki = 70 pM）。它含有一个约8埃的紧密重原子核心，这使其对电子显微镜实验非常有用。
