Blanco M, Coello J, González F, Iturriaga H, Maspoch S, Tomás X
Departamento de Química, Universidad Autónoma de Barcelona, Bellaterra, Spain.
J Pharm Biomed Anal. 1994 Apr;12(4):509-14. doi: 10.1016/0731-7085(93)e0004-7.
Partial least-squares calibration was used for the spectrophotometric determination of the active compound and preservative in a syrup also containing several light absorbing excipients. The calibration matrix was constructed from laboratory-made mixtures of the analytes and excipients and was used to quantify three samples from different batches. The most suitable conditions for quantitation were determined and the results obtained are compared with those provided by HPLC.
采用偏最小二乘法校准，用于分光光度法测定糖浆中活性成分和防腐剂，该糖浆还含有几种吸光辅料。校准矩阵由分析物和辅料的实验室自制混合物构建而成，并用于对来自不同批次的三个样品进行定量分析。确定了最适合定量分析的条件，并将所得结果与高效液相色谱法提供的结果进行比较。
