Bouskela E, Cyrino F Z
Department of Physiology and Biophysics, University of Lund, Sweden.
J Vasc Res. 1994 Sep-Oct;31(5):287-94. doi: 10.1159/000159055.
Intravital microscopy of the hamster cheek pouch microvasculature was used for in vivo studies on the effects of buflomedil, phentolamine (alpha-adrenergic receptor antagonist) and norepinephrine on the mean internal arteriolar diameter and spontaneous vasomotion. All drugs were applied topically. The vasomotor activity was studied in 125 arterioles (internal diameter range 18.0-62.0 microns) of 34 preparations. Addition of buflomedil (10(-9) to 10(-5) M) did not affect the arteriolar diameter significantly (from 100.7 +/- 3.5 to 106.4 +/- 1.8%, values expressed in percent of the initial diameter as mean +/- SE), but increased the vasomotion frequency and amplitude by approximately 20% (from 7.5 +/- 0.3 to 9.2 +/- 0.2 cpm) and 30% (from 7.3 +/- 0.3 to 10.0 +/- 0.5 micron), respectively. Phentolamine (10(-9) to 10(-5) M) dose-dependently increased the microvascular diameter (from 102.3 +/- 1.2 to 139.1 +/- 4.3%) and reduced the vasomotion frequency and amplitude (from 8.0 +/- 0.3 to 1.9 +/- 0.5 cpm and from 9.0 +/- 2.1 to 3.1 +/- 0.2 microns, respectively). Addition of buflomedil (10(-7) M) reduced the vasodilation evoked by phentolamine (from 103.3 +/- 0.7 to 127.0 +/- 1.5%) and potentiated its depressive effect on vasomotion frequency and amplitude (from 7.6 +/- 0.1 to 1.0 +/- 0.3 cpm and from 9.0 +/- 0.3 to 1.9 +/- 0.6 microns, respectively). Norepinephrine (10(-9) to 10(-5) M) dose-dependently decreased to arteriolar diameter (from 102.3 +/- 0.7 to 69.6 +/- 1.6%) and the vasomotion frequency and amplitude (from 8.4 +/- 0.2 to 0.4 +/- 0.3 cmp and from 8.7 +/- 0.2 to 0.5 +/- 0.4 microns, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
采用仓鼠颊囊微血管活体显微镜技术，对丁咯地尔、酚妥拉明（α - 肾上腺素能受体拮抗剂）和去甲肾上腺素对平均小动脉内径及自发性血管运动的影响进行体内研究。所有药物均局部应用。在34个标本的125条小动脉（内径范围为18.0 - 62.0微米）中研究血管运动活性。加入丁咯地尔（10⁻⁹至10⁻⁵M）对小动脉内径无显著影响（从初始内径的100.7±3.5%至106.4±1.8%，数值以初始内径的百分比表示，均值±标准误），但血管运动频率和幅度分别增加约20%（从7.5±0.3至9.2±0.2次/分钟）和30%（从7.3±0.3至10.0±0.5微米）。酚妥拉明（10⁻⁹至10⁻⁵M）剂量依赖性增加微血管直径（从102.3±1.2%至139.1±4.3%），并降低血管运动频率和幅度（分别从8.0±0.3至1.9±0.5次/分钟以及从9.0±2.1至3.1±0.2微米）。加入丁咯地尔（10⁻⁷M）可减弱酚妥拉明引起的血管舒张（从103.3±0.7%至127.0±1.5%），并增强其对血管运动频率和幅度的抑制作用（分别从7.6±0.1至1.0±0.3次/分钟以及从9.0±0.3至1.9±0.6微米）。去甲肾上腺素（10⁻⁹至10⁻⁵M）剂量依赖性减小小动脉内径（从102.3±0.7%至69.6±1.6%）以及血管运动频率和幅度（分别从8.4±0.2至0.4±0.3次/分钟以及从8.7±0.2至0.5±0.4微米）。（摘要截断于250字）
