Aterman K
Histochemistry. 1976 Oct 22;49(2):131-43. doi: 10.1007/BF00495677.
The historical development of the iodine-sulphuric acid reaction for amyloid is described. The reaction dates back to 1814 when Colin and Gaultier de Claubry, and independently Stromeyer, introduced the iodine reaction for starch. A variant of the acidified iodine reaction appears to have been used for printing paper by Gmelin in 1829, and in 1838 Schleiden used the iodine-sulphuric acid test on plants to demonstrate what he considered to be a transformation of the plant material into starch. Shortly afterwards Payen (1839) defined "cellulose", and the iodine-sulphuric acid reaction became a standard procedure used by botanists to demonstrate this plant component. In 1853 Virchow used Harting's (1847) procedure to demonstrate the reaction of Purkynĕ's corpora amylacea to this test, on the assumption that they might be cellulose derivatives, and applied it to what appeared to be similar corpuscles in a "waxy" spleen. The first histochemical reaction for amyloidosis had thus been introduced into pathology, and continued to exert from that time on an important influence on amyloid research, whose impact is felt to the present day.
本文描述了用于淀粉样蛋白的碘 - 硫酸反应的历史发展。该反应可追溯到1814年，当时科林和高尔捷·德·克劳布里，以及施特罗迈尔独立地引入了淀粉的碘反应。1829年，酸化碘反应的一种变体似乎已被格梅林用于纸张印刷，1838年施莱登对植物进行了碘 - 硫酸测试，以证明他认为的植物物质向淀粉的转化。不久之后，佩恩（1839年）定义了“纤维素”，碘 - 硫酸反应成为植物学家用于证明这种植物成分的标准程序。1853年，维尔肖采用哈廷（1847年）的方法，证明了浦肯野淀粉样体对该测试的反应，当时假定它们可能是纤维素衍生物，并将其应用于“蜡样”脾脏中看似相似的小体。淀粉样变性的首个组织化学反应由此被引入病理学，并从那时起继续对淀粉样蛋白研究产生重要影响，这种影响一直持续到今天。
