Russel M, Gold L, Morrissett H, O'Farrell P Z
J Biol Chem. 1976 Nov 25;251(22):7263-70.
Functional half-life measurements of the bacteriophage T4 gene 32 messenger RNA indicate that this mRNA is extremely stable. Regulation of gene 32 expression at the transcriptional level cannot account for the rapidity with which P32 synthesis can be repressed. Furthermore, derepression of P32 synthesis occurs in the presence of rifampicin, a drug which inhibits transcriptional initiation. In addition, T4-infected cultures in which P32 expression is repressed possess almost as much gene 32 mRNA as derepressed cultures. We conclude that expression of the T4 gene 32 protein is regulated at the level of translation.
噬菌体T4基因32信使核糖核酸的功能半衰期测量表明，这种信使核糖核酸极其稳定。基因32在转录水平上的表达调控无法解释P32合成被抑制的快速程度。此外，P32合成的去抑制在利福平存在的情况下发生，利福平是一种抑制转录起始的药物。另外，P32表达被抑制的T4感染培养物所拥有的基因32信使核糖核酸几乎与去抑制培养物一样多。我们得出结论，T4基因32蛋白质的表达是在翻译水平上受到调控的。
