通过抑制一种结构剪接增强子对RPL7B进行自动调节。

Autoregulation of RPL7B by inhibition of a structural splicing enhancer.

作者信息

Granas David, Hewa Ishan Gammadde, White Michael A, Stormo Gary D

机构信息

Department of Genetics and Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, United States.

出版信息

Nucleic Acids Res. 2025 Jul 19;53(14). doi: 10.1093/nar/gkaf739.

DOI:10.1093/nar/gkaf739

PMID:40744491

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12311795/

Abstract

Yeast ribosomal protein gene RPL7B is autoregulated by inhibition of splicing. The first intron has a "zipper stem" that brings the 5' splice site near the branch point (BP) and serves as an enhancer of splicing that is required for efficient splicing because the intron has a nonconsensus BP sequence of UGCUAAC. The intron also contains an alternative, and mutually exclusive, structure that is conserved across many yeast species. That conserved structure is a binding site for the Rpl7 protein so that when the protein is in excess over what is required for ribosomes, the protein binds to the conserved structure which eliminates the enhancer structure and represses splicing and gene expression.

摘要

酵母核糖体蛋白基因RPL7B通过抑制剪接进行自我调控。第一个内含子具有一个“拉链茎”，它将5'剪接位点带到分支点（BP）附近，并作为剪接增强子，由于该内含子具有非保守的UGCUAAC分支点序列，所以它是有效剪接所必需的。该内含子还包含一种在许多酵母物种中保守的替代且相互排斥的结构。该保守结构是Rpl7蛋白的结合位点，因此当该蛋白的量超过核糖体所需量时，该蛋白会与保守结构结合，从而消除增强子结构并抑制剪接和基因表达。