The P1 cloning system: past and future.

Suppr

超能文献

作者信息

Sternberg N

机构信息

DuPont Merck Pharmaceutical Company, Glenolden Laboratory, Pennsylvania 19036.

出版信息

Mamm Genome. 1994 Jul;5(7):397-404. doi: 10.1007/BF00356999.

DOI:10.1007/BF00356999

PMID:7919652

Abstract

摘要

相似文献

The P1 cloning system: past and future.

Mamm Genome. 1994 Jul;5(7):397-404. doi: 10.1007/BF00356999.

Genomic sequences with homology to the P element of Drosophila melanogaster occur in the blowfly Lucilia cuprina.

Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10753-7. doi: 10.1073/pnas.89.22.10753.

Targeting and retrofitting pre-existing libraries of transposon insertions with FRT and oriV elements for in-vivo generation of large quantities of any genomic fragment.利用FRT和oriV元件靶向改造现有的转座子插入文库，以便在体内大量生成任何基因组片段。

Gene. 1998 Nov 26;223(1-2):55-66. doi: 10.1016/s0378-1119(98)00410-7.

Using bacteriophage P1 system to clone high molecular weight genomic DNA.使用噬菌体P1系统克隆高分子量基因组DNA。

Methods Enzymol. 1992;216:549-74. doi: 10.1016/0076-6879(92)16049-p.

Construction of a PAC vector system for the propagation of genomic DNA in bacterial and mammalian cells and subsequent generation of nested deletions in individual library members.构建一种PAC载体系统，用于在细菌和哺乳动物细胞中扩增基因组DNA，并随后在单个文库成员中产生嵌套缺失。

Gene. 2001 Feb 7;264(1):11-8. doi: 10.1016/s0378-1119(01)00330-4.

Shotgun library construction in a day.

Biotechniques. 2003 Feb;34(2):244-6, 248, 250. doi: 10.2144/03342bm02.

Generation of a 50,000-member human DNA library with an average DNA insert size of 75-100 kbp in a bacteriophage P1 cloning vector.在噬菌体P1克隆载体中构建一个包含50000个成员的人类DNA文库，平均DNA插入片段大小为75 - 100千碱基对。

New Biol. 1990 Feb;2(2):151-62.

The P1 vector system for the preparation and screening of genomic libraries.

Genet Eng (N Y). 1994;16:213-28.

Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.用于革兰氏阴性菌DNA克隆的改良型广宿主质粒。

Gene. 1988 Oct 15;70(1):191-7. doi: 10.1016/0378-1119(88)90117-5.

Detection and characterization of transposable elements.

Methods Enzymol. 1993;224:322-34. doi: 10.1016/0076-6879(93)24024-o.

引用本文的文献

Cre recombinase mediated alterations of the mouse genome using embryonic stem cells.利用胚胎干细胞通过Cre重组酶介导对小鼠基因组进行改造。

Methods Mol Biol. 2008;461:111-32. doi: 10.1007/978-1-60327-483-8_8.

Genetic localization of a Drosophila melanogaster resistance gene to a parasitoid wasp and physical mapping of the region.黑腹果蝇对一种寄生蜂抗性基因的遗传定位及该区域的物理图谱绘制。

Genome Res. 1999 May;9(5):471-81.

Modification of bacterial artificial chromosome clones using Cre recombinase: introduction of selectable markers for expression in eukaryotic cells.利用Cre重组酶对细菌人工染色体克隆进行修饰：引入用于在真核细胞中表达的选择标记。

Genome Res. 1998 Apr;8(4):404-12. doi: 10.1101/gr.8.4.404.

Isolating large nested deletions in bacterial and P1 artificial chromosomes by in vivo P1 packaging of products of Cre-catalysed recombination between the endogenous and a transposed loxP site.通过对内源loxP位点与转座loxP位点之间的Cre催化重组产物进行体内P1包装，分离细菌人工染色体和P1人工染色体中的大型嵌套缺失。

Nucleic Acids Res. 1997 Jun 1;25(11):2205-12. doi: 10.1093/nar/25.11.2205.

本文引用的文献

Chromosome structure in phage t4, iii. Terminal redundancy and length determination.噬菌体T4中的染色体结构，iii. 末端冗余与长度测定

Proc Natl Acad Sci U S A. 1967 Feb;57(2):292-5. doi: 10.1073/pnas.57.2.292.

High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe.覆盖裂殖酵母粟酒裂殖酵母14 Mb基因组的高分辨率黏粒和P1图谱。

Cell. 1993 Apr 9;73(1):109-20. doi: 10.1016/0092-8674(93)90164-l.

Low-frequency chimeric yeast artificial chromosome libraries from flow-sorted human chromosomes 16 and 21.来自流式分选的人类16号和21号染色体的低频嵌合酵母人工染色体文库。

Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):1063-7. doi: 10.1073/pnas.90.3.1063.

Transgenic mice expressing high plasma concentrations of human apolipoprotein B100 and lipoprotein(a).表达高血浆浓度人载脂蛋白B100和脂蛋白(a)的转基因小鼠。

J Clin Invest. 1993 Dec;92(6):3029-37. doi: 10.1172/JCI116927.

Preparation and screening of an arrayed human genomic library generated with the P1 cloning system.用P1克隆系统构建的人基因组文库的制备及筛选

Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2629-33. doi: 10.1073/pnas.91.7.2629.

P1 and cosmid clones define the organization of 280 kb of the mouse H-2 complex containing the Cps-1 and Hsp70 loci.P1和黏粒克隆确定了包含Cps-1和Hsp70基因座的280 kb小鼠H-2复合体的组织结构。

Immunogenetics. 1994;39(1):48-55. doi: 10.1007/BF00171796.

Physical mapping of the rat tissue kallikrein family in two gene clusters by analysis of P1 bacteriophage clones.通过分析P1噬菌体克隆对大鼠组织激肽释放酶家族在两个基因簇中的物理图谱进行研究。

Genomics. 1994 Jul 15;22(2):404-17. doi: 10.1006/geno.1994.1402.

Genetic and physical map of a P1 miniplasmid.一个P1小质粒的遗传图谱和物理图谱。

J Bacteriol. 1982 Oct;152(1):63-71. doi: 10.1128/jb.152.1.63-71.1982.

Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.克隆编码枯草芽孢杆菌外切酶果聚糖蔗糖酶的结构基因sacB：该基因在大肠杆菌中的表达。

J Bacteriol. 1983 Mar;153(3):1424-31. doi: 10.1128/jb.153.3.1424-1431.1983.

Studies on the properties of P1 site-specific recombination: evidence for topologically unlinked products following recombination.P1位点特异性重组特性的研究：重组后拓扑学上不相连产物的证据。

Cell. 1983 Apr;32(4):1301-11. doi: 10.1016/0092-8674(83)90311-2.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验