P1和黏粒克隆确定了包含Cps-1和Hsp70基因座的280 kb小鼠H-2复合体的组织结构。

P1 and cosmid clones define the organization of 280 kb of the mouse H-2 complex containing the Cps-1 and Hsp70 loci.

作者信息

Gasser D L, Sternberg N L, Pierce J C, Goldner-Sauve A, Feng H, Haq A K, Spies T, Hunt C, Buetow K H, Chaplin D D

机构信息

Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104.

出版信息

Immunogenetics. 1994;39(1):48-55. doi: 10.1007/BF00171796.

DOI:10.1007/BF00171796

PMID:7993390

Abstract

A 280 kilobase (kb) contig was isolated from mouse genomic P1 and cosmid libraries, using as probes human cDNA and genomic DNA fragments that map in the interval between the second component of complement and tumor necrosis factor genes of the HLA complex. The clone contig demonstrates synteny of eleven mouse genes that are homologous to genes initially mapped within the human major histocompatibility complex. These include the mouse homologs of BAT2 (HLA-B-associated transcript 2) through BAT9 and also three HSP70-related genes. Five P1 clones form a contig of 240 kb that spans from BAT9 through BAT3. Twelve cosmid clones are arranged in three contigs that confirm most of the structure of the P1 contig and link the mouse BAT3 homolog to the BAT2 homolog approximately 15 kb farther telomeric. Polymorphic DNA markers within the cloned region were used to map the cleft palate susceptibility-1 (Cps-1) locus to the interval between Hsp70.1 and BAT6 (valyl-tRNA synthetase). This refines the location of the Cps-1 locus to a 45 kb region contained in the H2-124 P1 insert.

摘要

从小鼠基因组P1和黏粒文库中分离出一个280千碱基对（kb）的重叠群，使用定位在HLA复合体补体第二成分和肿瘤坏死因子基因之间区间的人类cDNA和基因组DNA片段作为探针。该克隆重叠群显示了11个小鼠基因的同线性，这些基因与最初定位在人类主要组织相容性复合体中的基因同源。其中包括BAT2（HLA - B相关转录本2）到BAT9的小鼠同源物，以及三个与HSP70相关的基因。五个P1克隆形成了一个240 kb的重叠群，从BAT9延伸到BAT3。十二个黏粒克隆排列成三个重叠群，证实了P1重叠群的大部分结构，并将小鼠BAT3同源物与BAT2同源物在端粒方向大约再远15 kb处相连。克隆区域内的多态性DNA标记用于将腭裂易感性-1（Cps-1）基因座定位到Hsp70.1和BAT6（缬氨酰-tRNA合成酶）之间的区间。这将Cps-1基因座的位置精确到H2 - 124 P1插入片段中包含的一个45 kb区域。