Acosta A, Sarmiento M E, González A, Estévez P, Aguila A, Infante J F, Izquierdo L, Capó V, Sierra G, Malberty J A
Arch Med Res. 1994 Summer;25(2):159-63.
With the aim of determining the distribution and humoral immunogenicity of the bacillus Calmette-Guérin (BCG) administered by the oral (O), intravenous (IV) and subcutaneous (SC) routes, we studied 54 male Balb/c mice weighing 17-22 g that had been inoculated with BCG (10(6) CFU) by the O (n = 18), IV (n = 18) and SC (n = 18) routes. At weekly intervals we determined the distribution of the microorganism using histopathological techniques including Ziehl-Neelsen staining. Serum samples of the same animals were analyzed by ELISA and Western blot to determine the antibody response to the microorganism. In all groups, distinctive histopathologic lesions harboring the microorganism were found. Using the SC route the lesions were located at the inoculation site, whereas there was systemic dissemination with the O and IV routes, being more prominent with the latter. Anti-BCG antibodies were detected by ELISA in all groups; this response was more intense in the IV group, followed by the SC and O groups. In the Western blot analysis, reactivity against multiple bands and the predominant recognition of a 65 kd band in all groups was observed.
为了确定经口服（O）、静脉注射（IV）和皮下（SC）途径接种的卡介苗（BCG）的分布及体液免疫原性，我们研究了54只体重17 - 22克的雄性Balb/c小鼠，它们分别经口服（n = 18）、静脉注射（n = 18）和皮下（n = 18）途径接种了卡介苗（10⁶CFU）。我们每周使用包括齐-尼氏染色在内的组织病理学技术来确定微生物的分布。通过ELISA和蛋白质免疫印迹法分析同一批动物的血清样本，以确定对该微生物的抗体反应。在所有组中，均发现了含有该微生物的独特组织病理学病变。采用皮下途径时，病变位于接种部位，而经口服和静脉注射途径则出现全身播散，后者更为显著。通过ELISA在所有组中均检测到了抗卡介苗抗体；该反应在静脉注射组中更为强烈，其次是皮下注射组和口服组。在蛋白质免疫印迹分析中，观察到所有组均对多条条带产生反应，且主要识别一条65 kd的条带。
