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核酸中主链扭转角ε的测定。

Determination of the backbone torsion angle epsilon in nucleic acids.

作者信息

Blommers M J, Nanz D, Zerbe O

机构信息

Department of Physics, Ciba-Geigy AG, Basel, Switzerland.

出版信息

J Biomol NMR. 1994 Sep;4(5):595-601. doi: 10.1007/BF00404271.

DOI:10.1007/BF00404271
PMID:7919949
Abstract

The multiplet structure of cross peaks in double-quantum-filtered COSY NMR spectra is analysed for those resonances that include passive heteronuclear couplings. Interestingly, the cross peak involving the sugar-ring protons H2' and H3' in nucleic acids display an E. COSY-type appearance exclusively when the backbone torsion angle epsilon (C4'-C3'-O3'-P) adopts a gauche(-) conformation. This observation allows an unambiguous analysis of the conformation around epsilon, without the knowledge of 3JCP coupling constants.

摘要

对双量子滤波COSY NMR谱中包含被动异核耦合的那些共振峰的交叉峰多重结构进行了分析。有趣的是，当主链扭转角ε（C4'-C3'-O3'-P）采取gauche(-)构象时，核酸中涉及糖环质子H2'和H3'的交叉峰仅呈现出E-COSY型外观。这一观察结果使得在无需了解3JCP耦合常数的情况下，能够对ε周围的构象进行明确分析。

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本文引用的文献

1
The solution structure of the circular trinucleotide cr(GpGpGp) determined by NMR and molecular mechanics calculation.通过核磁共振（NMR）和分子力学计算确定的环状三核苷酸cr(GpGpGp)的溶液结构。
Nucleic Acids Res. 1994 Jul 11;22(13):2658-66. doi: 10.1093/nar/22.13.2658.
2
Effects of the introduction of L-nucleotides into DNA. Solution structure of the heterochiral duplex d(G-C-G-(L)T-G-C-G).d(C-G-C-A-C-G-C) studied by NMR spectroscopy.L-核苷酸引入DNA的效应。通过核磁共振光谱研究的异手性双链体d(G-C-G-(L)T-G-C-G).d(C-G-C-A-C-G-C)的溶液结构。
Biochemistry. 1994 Jun 28;33(25):7886-96. doi: 10.1021/bi00191a016.
3
Conformation of the circular dumbbell d<pCGC-TT-GCG-TT>: structure determination and molecular dynamics.
环状哑铃状d<pCGC-TT-GCG-TT>的构象：结构测定与分子动力学
J Biomol NMR. 1995 Dec;6(4):403-22. doi: 10.1007/BF00197639.
Carbon-13 NMR in conformational analysis of nucleic acid fragments. 2. A reparametrization of the Karplus equation for vicinal NMR coupling constants in CCOP and HCOP fragments.
碳-13核磁共振在核酸片段构象分析中的应用。2. CCOP和HCOP片段中邻位核磁共振耦合常数的Karplus方程的重新参数化。
J Biomol Struct Dyn. 1984 Jun;1(6):1387-405. doi: 10.1080/07391102.1984.10507527.
4
Restrained refinement of the monoclinic form of yeast phenylalanine transfer RNA. Temperature factors and dynamics, coordinated waters, and base-pair propeller twist angles.酵母苯丙氨酸转移核糖核酸单斜晶型的约束精修。温度因子与动力学、配位水及碱基对螺旋桨扭转角
Biochemistry. 1986 Aug 26;25(17):4868-78. doi: 10.1021/bi00365a022.
5
Nucleic acids and nuclear magnetic resonance.核酸与核磁共振。
Eur J Biochem. 1988 Dec 1;178(1):1-38. doi: 10.1111/j.1432-1033.1988.tb14425.x.
6
Solution structure of the 3'-5' cyclic dinucleotide d(pApA). A combined NMR, UV melting, and molecular mechanics study.
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7
The three-dimensional structure of a DNA hairpin in solution two-dimensional NMR studies and structural analysis of d(ATCCTATTTATAGGAT).溶液中DNA发夹的三维结构——d(ATCCTATTTATAGGAT)的二维核磁共振研究与结构分析
Eur J Biochem. 1991 Oct 1;201(1):33-51. doi: 10.1111/j.1432-1033.1991.tb16253.x.
8
Determination of heteronuclear long-range couplings to heteronuclei in natural abundance by two- and three-dimensional NMR spectroscopy.通过二维和三维核磁共振波谱法测定天然丰度下与异核的异核远程耦合。
J Biomol NMR. 1991 Nov;1(4):403-20. doi: 10.1007/BF02192863.