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1
Thermodynamics of stacking and of self-association of the dinucleoside monophosphate m2(6)A-U from proton NMR chemical shifts: differential concentration temperature profile method.基于质子核磁共振化学位移的二磷酸单核苷m2(6)A-U堆积与自缔合的热力学:差分浓度-温度分布法
Eur J Biochem. 1982 Dec 15;129(2):343-57. doi: 10.1111/j.1432-1033.1982.tb07057.x.
2
Carbon-13 NMR in conformational analysis of nucleic acid fragments. Heteronuclear chemical shift correlation spectroscopy of RNA constituents.核酸片段构象分析中的碳-13核磁共振。RNA成分的异核化学位移相关光谱学。
Nucleic Acids Res. 1983 Oct 25;11(20):7215-30. doi: 10.1093/nar/11.20.7215.
3
Advanced nuclear magnetic resonance lanthanide probe analyses of short-range conformational interrelations controlling ribonucleic acid structures.用于分析控制核糖核酸结构的短程构象相互关系的先进核磁共振镧系元素探针分析
Biochemistry. 1981 May 12;20(10):2981-8. doi: 10.1021/bi00513a041.

核酸片段构象分析中的碳-13核磁共振。3. 根据CCOP和HCOP核磁共振耦合常数确定d(TpA)中扭转角ε的大小。

Carbon-13 NMR in conformational analysis of nucleic acid fragments. 3. The magnitude of torsional angle epsilon in d(TpA) from CCOP and HCOP NMR coupling constants.

作者信息

Lankhorst P P, Haasnoot C A, Erkelens C, Altona C

出版信息

Nucleic Acids Res. 1984 Jul 11;12(13):5419-28. doi: 10.1093/nar/12.13.5419.

DOI:10.1093/nar/12.13.5419
PMID:6087285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318928/
Abstract

Carbon-13 NMR spectra of the deoxyribonucleotide d(TpA), 3',5'-cyclic AMP and 3',5'-cyclic dAMP were measured. It is shown that the different substitution of C2' in deoxyribonucleotides versus ribonucleotides does not affect the vicinal C2'-C3'-O3'-P coupling to a measurable extent. Therefore, the same set of Karplus parameters may be used for the C2'-C3-O3'-P couplings in ribonucleotides and in deoxyribonucleotides. Vicinal carbon-phosphorus and proton-phosphorus coupling constants are used to calculate the magnitude of the torsion angle epsilon (C4'-C3'-O3'-P), which amounts to 195(0) in the trans conformer and to 261(0) in the gauche(-) conformer.

摘要

测量了脱氧核糖核苷酸d(TpA)、3',5'-环磷酸腺苷和3',5'-环磷酸脱氧腺苷的碳-13核磁共振谱。结果表明,脱氧核糖核苷酸与核糖核苷酸中C2'的不同取代对邻位C2'-C3'-O3'-P偶合的影响在可测量范围内不明显。因此,核糖核苷酸和脱氧核糖核苷酸中C2'-C3-O3'-P偶合可使用同一组卡普拉斯参数。利用邻位碳-磷和质子-磷偶合常数计算扭转角ε(C4'-C3'-O3'-P)的大小,反式构象中该值为195(0),gauche(-)构象中为261(0)。