Webb T E, Simon J, Bateson A N, Barnard E A
Molecular Neurobiology Unit, Royal Free Hospital School of Medicine, London, United Kingdom.
Cell Mol Biol (Noisy-le-grand). 1994 May;40(3):437-42.
Extracellular ATP acts at specific cell surface purinoceptors to elicit a wide range of physiological responses. We have recently isolated a cDNA for a G-protein-coupled P2 purinoceptor (P2y1) from chick brain. It has been defined as a P2Y-like purinoceptor by the rank order of potency of P2 purinoceptor ligands, determined electrophysiologically in the Xenopus oocyte expression system. Here, we examine the ligand selectivity of this recombinant receptor, expressed transiently in COS-7 cells. The regional distribution of the P2y1 purinoceptor transcript within the one-day-post-hatch chick brain was also determined. It is widely expressed in the cerebellum and telencephalon and in specific nuclei of the mesencephalon and diencephalon, suggesting a neuronal localization of the P21 purinoceptor.
细胞外ATP作用于特定的细胞表面嘌呤受体，引发多种生理反应。我们最近从鸡脑中分离出一种G蛋白偶联P2嘌呤受体（P2y1）的cDNA。通过在非洲爪蟾卵母细胞表达系统中进行电生理测定的P2嘌呤受体配体的效价顺序，它被定义为一种P2Y样嘌呤受体。在这里，我们研究了这种在COS-7细胞中瞬时表达的重组受体的配体选择性。还确定了孵化后一天的鸡脑内P2y1嘌呤受体转录本的区域分布。它在小脑、端脑以及中脑和间脑的特定核团中广泛表达，表明P21嘌呤受体定位于神经元。
