Huits H S, Gerats A G, Kreike M M, Mol J N, Koes R E
Department of Genetics, Vrije Universiteit, Amsterdam, The Netherlands.
Plant J. 1994 Sep;6(3):295-310. doi: 10.1046/j.1365-313x.1994.06030295.x.
The functions of four loci (An1, An2, An4, and An6) which control pigmentation in flowers of Petunia hybrida have been characterized. Linkage-analysis and molecular complementation experiments showed that the An6 locus contains the structural dfrA gene, encoding the enzyme dihydroflavonol 4-reductase (DFR). Analysis of gus gene expression driven by the dfrA promoter in transgenic plants showed that the dfrA promoter is highly active in the flower corolla, the anthers and seeds and, at a lower level, in ovules and the flower stem. These data are discussed in relation to the expression of other pigmentation genes and the accumulation pattern of anthocyanins. The expression of the drfA-gus transgene was dependent on the genes an1 (in every tissue), an2 (in the flower limb only) and an4(in anthers), demonstrating that these genes encode regulatory factors that control drfA promoter activity.
已对矮牵牛花朵中控制色素沉着的四个基因座（An1、An2、An4和An6）的功能进行了表征。连锁分析和分子互补实验表明，An6基因座包含结构dfrA基因，该基因编码二氢黄酮醇4-还原酶（DFR）。对转基因植物中由dfrA启动子驱动的gus基因表达的分析表明，dfrA启动子在花的花冠、花药和种子中高度活跃，在胚珠和花茎中的活性较低。结合其他色素沉着基因的表达和花青素的积累模式对这些数据进行了讨论。drfA-gus转基因的表达依赖于an1基因（在每个组织中）、an2基因（仅在花边缘）和an4基因（在花药中），这表明这些基因编码控制drfA启动子活性的调控因子。
