Helariutta Y, Kotilainen M, Elomaa P, Teeri T H
Institute of Biotechology, University of Helsinki, Finland.
Plant Mol Biol. 1995 Aug;28(5):935-41. doi: 10.1007/BF00042077.
In the ornamental cut flower plant Gerbera hybrida the spatial distribution of regulatory molecules characteristic of differentiation of the composite inflorescence is visualized as the various patterns of anthocyanin pigmentation of different varieties. In order to identify genes that the plant can regulate according to these anatomical patterns, we have analysed gene expression affecting two enzymatic steps, chalcone synthase (CHS) and dihydroflavonol-4-reductase (DFR), in five gerbera varieties with spatially restricted anthocyanin pigmentation patterns. The dfr expression profiles vary at the levels of floral organ, flower type and region within corolla during inflorescence development according to the anthocyanin pigmentation of the cultivars. In contrast, chs expression, although regulated in a tissue-specific manner during inflorescence development, varies only occasionally. The variation in the dfr expression profiles between the varieties reveals spatially specific gene regulation that senses the differentiation events characteristic of the composite inflorescence.
在观赏切花植物非洲菊中，复合花序分化特征的调控分子的空间分布表现为不同品种花青素色素沉着的各种模式。为了鉴定植物能够根据这些解剖学模式进行调控的基因，我们分析了五个具有空间受限花青素色素沉着模式的非洲菊品种中影响两个酶促步骤的基因表达，这两个酶促步骤分别是查尔酮合酶（CHS）和二氢黄酮醇-4-还原酶（DFR）。在花序发育过程中，根据品种的花青素色素沉着情况，dfr的表达谱在花器官、花型和花冠内区域的水平上有所不同。相比之下，chs的表达虽然在花序发育过程中以组织特异性方式受到调控，但只是偶尔发生变化。品种间dfr表达谱的差异揭示了感知复合花序分化事件的空间特异性基因调控。
