Hagihara M, Shimura T, Takebe K, Munkhbat B, Tsuji K
Department of Transplantation Immunology, University School of Medicine, Kanagawa, Japan.
Cell Transplant. 1994 Jul-Aug;3(4):283-90. doi: 10.1177/096368979400300404.
Isogeneic (rat) and xenogeneic (swine) fetal liver fragments (FLF) transplantation into the omentum was performed for D-galactosamine (D-Gal)-induced acute and carbon tetrachloride (CCl4)-induced chronic hepatic failure in rats. The recipients that had iso or xeno FLF showed higher survival rates than the nontransplanted controls on a lethal dose (2.6 g/kg body weight) of D-Gal (survival rates: Iso 70%, Xeno 80%, and control 9.1%). On a sublethal dose (1.0 or 1.2 g/kg) of D-Gal, iso, or xeno FLF caused marked improvement of the values of GPT, GOT, and total bilirubin (T.Bil); at 72 h after D-Gal injection they went significantly lower than those of controls (Iso vs. control; p < 0.01, Xeno vs. control; p < 0.05). Histological examination of the livers revealed severe damage in controls, however, only a slight damage was found in iso or xeno FLF transplanted rats. Iso grafts were fairly well preserved in the omentum at 72 h posttransplants, however, xeno graft had almost changed into a necrotic tissue. CCl4 was administered subcutaneously for 14 wk to induce chronic hepatic failure and then iso FLF were transplanted 3 days after the last CCl4 injection. Iso FLF transplanted rats showed higher improvement of GPT and GOT values at 12 days posttransplants compared with controls (GPT p < 0.01, GOT p < 0.05), although histological improvement was not so remarkable in both group. Iso grafts formed nodules with many hepatocytes in the omentum 12 days posttransplant. The results indicate that iso or xeno FLF transplantation could be an alternative approach for incurable liver insufficiencies.
将同基因（大鼠）和异种（猪）胎儿肝碎片（FLF）移植到大网膜，用于治疗D - 半乳糖胺（D - Gal）诱导的大鼠急性肝衰竭和四氯化碳（CCl4）诱导的大鼠慢性肝衰竭。接受同基因或异种FLF移植的大鼠在给予致死剂量（2.6 g/kg体重）的D - Gal后，其存活率高于未移植的对照组（存活率：同基因70%，异种80%，对照组9.1%）。在给予亚致死剂量（1.0或1.2 g/kg）的D - Gal时，同基因或异种FLF可使谷丙转氨酶（GPT）、谷草转氨酶（GOT）和总胆红素（T.Bil）值显著改善；在注射D - Gal后72小时，这些值显著低于对照组（同基因与对照组比较；p < 0.01，异种与对照组比较；p < 0.05）。肝脏组织学检查显示，对照组肝脏有严重损伤，然而，在接受同基因或异种FLF移植的大鼠中仅发现轻微损伤。同基因移植物在移植后72小时在大网膜中保存良好，然而，异种移植物几乎已变成坏死组织。皮下注射CCl4 14周以诱导慢性肝衰竭，然后在最后一次注射CCl4后3天移植同基因FLF。与对照组相比，移植同基因FLF的大鼠在移植后12天GPT和GOT值有更高程度的改善（GPT p < 0.01，GOT p < 0.05），尽管两组的组织学改善都不显著。移植后12天，同基因移植物在大网膜中形成含有许多肝细胞的结节。结果表明，同基因或异种FLF移植可能是治疗不可治愈性肝衰竭的一种替代方法。
