Kanai N, Morita N, Munkhbat B, Gansuvd B, Hagihara M, Nagamachi Y, Tsuji K
Department of Transplantation Immunology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Cell Transplant. 1998 Jul-Aug;7(4):417-20. doi: 10.1177/096368979800700413.
Transplantation of xeno fetal liver fragments (FLF) could be an alternative or supplementary therapy for acute and chronic liver failure not resolved by routine medical therapies. However, the xenografts themselves are rejected by the host immune system. To overcome these problems, immunoisolate capsules with various cutoff points, from 50,000 (YM30) to 500,000 (ZM500) were tested for their protective effects on FLF graft survival. In an in vitro study, the capsule with the smallest cutoff size (YM30) had an excellent protective effect on the grafts it contained, and showed the lowest GOT values in the culture supernatant and the normal histological structure. In an in vivo study using rats, the same capsule enabled a FLF graft to survive as long as 21 days, even with severe IgG deposition on and within the graft. In another in vivo study, which used beagle dog, however, it did not improve the natural course of survival of the graft, which had totally degenerated by day 7. In conclusion, 1) Immunocapsules, especially those with the smallest cutoff values, impeded the infiltration of the (xeno) humoral attacking factor, but the blocking effect was not complete, as shown by the immunoglobulin (IgG) deposit on the grafts they contained. 2) The FLFs with capsules survived longer than those without capsules--only in rats, not in beagles. This difference may be attributable to the difference of the extent of humoral or nutritional response to the xenografts.
移植异种胎儿肝碎片（FLF）可能是一种针对常规药物治疗无法解决的急慢性肝衰竭的替代或补充疗法。然而，异种移植物本身会被宿主免疫系统排斥。为克服这些问题，对各种截留分子量从50,000（YM30）到500,000（ZM500）的免疫隔离胶囊进行了测试，以考察其对FLF移植物存活的保护作用。在一项体外研究中，截留尺寸最小的胶囊（YM30）对其包裹的移植物具有出色的保护作用，并且在培养上清液中显示出最低的谷草转氨酶（GOT）值以及正常的组织结构。在一项使用大鼠的体内研究中，即使移植物上和移植物内有严重的IgG沉积，相同的胶囊也能使FLF移植物存活长达21天。然而，在另一项使用比格犬的体内研究中，它并未改善移植物的自然存活过程，移植物在第7天已完全退化。总之，1）免疫胶囊，尤其是截留值最小的那些，可阻碍（异种）体液攻击因子的浸润，但如包裹的移植物上有免疫球蛋白（IgG）沉积所示，这种阻断作用并不完全。2）带有胶囊的FLF比没有胶囊的FLF存活时间更长——仅在大鼠中如此，在比格犬中并非如此。这种差异可能归因于对异种移植物的体液或营养反应程度的不同。
