Broxmeyer H E, Carow C E
Walther Oncology Center, Indiana University, Indianapolis 46202-5121.
J Hematother. 1993 Summer;2(2):197-9. doi: 10.1089/scd.1.1993.2.197.
Based on in vitro studies, umbilical-cord blood has been used clinically to engraft the hematopoietic systems of children. To evaluate the capacity of a single collection of cord blood to engraft an adult, it is important to consider not only the number, but also the quality of stem/progenitor cells in cord blood. We demonstrate that cord blood contains multipotential progenitor cells, possibly subsets of stem cells, that have extensive self-renewal capacity, as estimated by their capacity to form numerous secondary colonies of multiple lineages in secondary replates from single multipotential colonies. This replating capacity is enhanced by a factor or factors, perhaps novel, in cord blood plasma.
基于体外研究，脐带血已在临床上用于移植儿童的造血系统。为了评估单次采集的脐带血移植成人造血系统的能力，不仅要考虑脐带血中干/祖细胞的数量，还要考虑其质量。我们证明，脐带血含有多能祖细胞，可能是干细胞的亚群，具有广泛的自我更新能力，这是通过它们在来自单个多能集落的二次培养板中形成多个谱系的大量二次集落的能力来估计的。脐带血血浆中的一种或多种因素（可能是新的因素）增强了这种再接种能力。
