Gadamski R, Kroh H
Department of Neuropathology, Polish Academy of Sciences, Warsaw.
Folia Neuropathol. 1994;32(2):95-9.
The study was performed on 98 Mongolian gerbils. Cerebral ischemia was evoked by ligation of both common carotid arteries for 5 min. After five postischemic days the animals were decapitated, brains fixed, paraffin section stained with histological methods and for GFAP with ABC method. The investigated animals (ca 18%) presented asymmetrical morphological lesions of the CA1 hippocampal sector showing irregular loss of neurons in both cerebral hemispheres. GFAP immunostaining demonstrated various astroglial proliferation in asymmetrical lesions of CA1 sector. In partial injury to CA1 neurons the astrocytes in stratum (s) pyramidale were single but their number still increased when total loss of pyramidal neurons occurred. Except s. lacunosum moleculare the number of GFAP-positive astrocytes in the remaining layers of dorsal hippocampus presented a direct relationship with the intensity of morphological changes and was highest when 70% loss of pyramidal cells was observed.
该研究对98只蒙古沙鼠进行。通过结扎双侧颈总动脉5分钟诱发脑缺血。缺血后5天，将动物断头，固定大脑，用组织学方法进行石蜡切片染色，并用ABC法对胶质纤维酸性蛋白（GFAP）进行染色。所研究的动物（约18%）呈现海马CA1区不对称形态学损伤，表现为双侧大脑半球神经元不规则缺失。GFAP免疫染色显示CA1区不对称损伤中有各种星形胶质细胞增生。在CA1神经元部分损伤时，锥体细胞层的星形胶质细胞单个存在，但当锥体细胞完全缺失时其数量仍会增加。除分子层外，背侧海马其余层中GFAP阳性星形胶质细胞的数量与形态学变化强度呈直接关系，在观察到锥体细胞丢失70%时最高。
