Kroh H, Gadamski R
Department of Neuropathology, Polish Academy of Sciences, Warszawa.
Folia Neuropathol. 1995;33(3):153-7.
Twenty two Mongolian gerbils after 5 min bilateral carotid artery occlusion and 6, 12, 24, 48, 72, 94 hours and 5 days survival were investigated for the neuronal changes in dorsal hippocampus. Paraffin sections were stained with cresyl-violet and marked by their binding of Concanavalin A (Con A) labelled with peroxidase. The degeneration and neuronal loss was observed only in CA1 sectors in almost all experimental groups, whereas the decreased binding of Con A by the neurons of CA1 sector corresponded to the intensity of histologic changes but appeared also only in this sector even without any histological changes. These observation can point at the subthreshold damage of CA1 neurons as result of either diminished supply or increased metabolism of d-glucose, or diminished number of Con A receptors or changes of their specificity after ischemic period.
对22只蒙古沙鼠进行双侧颈动脉闭塞5分钟后，分别在存活6、12、24、48、72、94小时和5天进行研究，观察其背侧海马体的神经元变化。石蜡切片用甲酚紫染色，并用过氧化物酶标记的伴刀豆球蛋白A（Con A）结合进行标记。几乎在所有实验组中，仅在CA1区观察到变性和神经元丢失，而CA1区神经元Con A结合减少与组织学变化的强度相对应，并且即使在没有任何组织学变化的情况下也仅在该区域出现。这些观察结果表明，缺血期后，CA1神经元可能因d - 葡萄糖供应减少或代谢增加、Con A受体数量减少或其特异性改变而受到阈下损伤。
