Jin G R, Rao Z R, Shi J W
Department of Anatomy, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
Brain Res. 1994 Jun 20;648(2):196-202. doi: 10.1016/0006-8993(94)91118-5.
Horseradish peroxidase (HRP) was stereotaxically injected into the nucleus accumbens (Acb), and visceral noxious stimulation given by injecting formalin into the stomach. Sections of the medulla were subjected to HRP reaction combined with immunohistochemical reactions for Fos protein (ABC method) and tyrosine hydroxylase (TH, PAP method). The catecholaminergic neurons of the medulla (including vagal complex, ventrolateral medulla and reticular formation between them) which expressed Fos protein and projected to Acb were studied. The results showed that HRP retrogradely labeled cells were seen in the medulla bilaterally with apparent ipsilateral predominance and TH-LI and Fos-LI single labeled cells were bilaterally distributed; HRP/TH,TH/Fos double labeled neurons were more numerous than HRP/Fos double-labeled neurons. HRP/TH/Fos triple-labeled neurons were small in number and were mainly distributed in the nucleus tractus solitarii (nTS) and ventrolateral medulla (VLM), but only a few labeled cells were located in RF between nTS and VLM. It is concluded that TH-LI neurons in the medulla projected to Acb and some of them expressed Fos protein after noxious stimulation of the stomach.
将辣根过氧化物酶（HRP）立体定向注射到伏隔核（Acb），并通过向胃内注射福尔马林给予内脏伤害性刺激。对延髓切片进行HRP反应，并结合Fos蛋白免疫组化反应（ABC法）和酪氨酸羟化酶（TH，PAP法）。研究了延髓中表达Fos蛋白并投射到Acb的儿茶酚胺能神经元（包括迷走复合体、延髓腹外侧和它们之间的网状结构）。结果显示，双侧延髓均可见HRP逆行标记细胞，同侧明显占优势，TH-LI和Fos-LI单标记细胞双侧分布；HRP/TH、TH/Fos双标记神经元比HRP/Fos双标记神经元更多。HRP/TH/Fos三标记神经元数量较少，主要分布在孤束核（nTS）和延髓腹外侧（VLM），但仅少数标记细胞位于nTS和VLM之间的网状结构（RF）中。结论是，延髓中的TH-LI神经元投射到Acb，其中一些在胃受到伤害性刺激后表达Fos蛋白。
