Dong Y X, Xiong K H, Rao Z R
Department of Anatomy, Fourth Military Medical University, P.R. China.
J Hirnforsch. 1997;38(1):3-7.
Catecholaminergic neurons in the medulla projecting to lateral hypothalamic area (LH) and expressing Fos were investigated in the rat by a triple labelling method, in which horseradish peroxidase (HRP) was injected into the LH, and visceral noxious stimulation was induced by formalin injection into the stomach, sections of the medulla were stained histochemically for HRP and immunohistochemically for Fos and tyrosine hydroxylase (TH). Some neurons labeled with HRP and showing both Fos- and TH-like immunoreactivities were mainly found in the nucleus tractus solitarii (NTS) and ventrolateral medulla (VLM) at the middle and caudal levels of medulla, only a few of them were located in the reticular formation between NTS and VLM. These results indicated that some medullary catecholaminergic neurons projected to the LH and some of them might be involved in the transmissing processes of stress responses to the visceral nociceptive information produced by chemical stimulation.
采用三重标记法在大鼠中研究了投射至下丘脑外侧区（LH）并表达Fos的延髓儿茶酚胺能神经元，该方法是将辣根过氧化物酶（HRP）注入LH，并通过向胃内注射福尔马林诱导内脏伤害性刺激，对延髓切片进行HRP组织化学染色以及Fos和酪氨酸羟化酶（TH）免疫组织化学染色。一些被HRP标记且同时显示Fos样和TH样免疫反应性的神经元主要位于延髓中、尾段的孤束核（NTS）和延髓腹外侧区（VLM），只有少数位于NTS和VLM之间的网状结构中。这些结果表明，一些延髓儿茶酚胺能神经元投射至LH，其中一些可能参与了对化学刺激产生的内脏伤害性信息的应激反应传递过程。
