神经胶质细胞外基质调节培养的牛脑微血管和牛主动脉内皮细胞中的γ-谷氨酰转肽酶活性。

Glial extracellular matrix modulates gamma-glutamyl transpeptidase activity in cultured bovine brain capillary and bovine aortic endothelial cells.

作者信息

Mizuguchi H, Hashioka Y, Fujii A, Utoguchi N, Kubo K, Nakagawa S, Baba A, Mayumi T

机构信息

Faculty of Pharmaceutical Science, Osaka University, Japan.

出版信息

Brain Res. 1994 Jul 18;651(1-2):155-9. doi: 10.1016/0006-8993(94)90692-0.

DOI:10.1016/0006-8993(94)90692-0

PMID:7922563

Abstract

Glial extracellular matrix (ECM) elevated gamma-glutamyl transpeptidase (gamma-GTP) activity in cultured bovine brain capillary and aortic endothelial cells (BBCEC, BAEC). In particular, the ECM of glial cells cultured with the conditioned medium of BAEC (BAEC CM) dramatically elevated gamma-GTP activity in BBCEC and BAEC. The ECM of glial cells cultured with BBCEC CM also had a marked effect. The ECM of 3T3 cells cultured with BAEC CM, and the ECM of glial cells cultured with 3T3 CM had no effect. Glial CM had no effect on gamma-GTP activity in BBCEC and BAEC. These findings indicate that gamma-GTP activity in endothelial cells (EC) is modulated by glial ECM, and that the factor of ECM that affects gamma-GTP activity in EC arises from the interaction between glial cells and EC.

摘要

神经胶质细胞外基质（ECM）可提高培养的牛脑微血管和主动脉内皮细胞（BBCEC、BAEC）中的γ-谷氨酰转肽酶（γ-GTP）活性。特别是，用BAEC条件培养基（BAEC CM）培养的神经胶质细胞的ECM显著提高了BBCEC和BAEC中的γ-GTP活性。用BBCEC CM培养的神经胶质细胞的ECM也有显著作用。用BAEC CM培养的3T3细胞的ECM以及用3T3 CM培养的神经胶质细胞的ECM没有作用。神经胶质细胞CM对BBCEC和BAEC中的γ-GTP活性没有影响。这些发现表明，内皮细胞（EC）中的γ-GTP活性受神经胶质细胞ECM调节，且影响EC中γ-GTP活性的ECM因子源于神经胶质细胞与EC之间的相互作用。