分离的脑内皮细胞中的γ-谷氨酰转肽酶：体外胶质细胞诱导作用

gamma-Glutamyl transpeptidase in isolated brain endothelial cells: induction by glial cells in vitro.

作者信息

DeBault L E, Cancilla P A

出版信息

Science. 1980 Feb 8;207(4431):653-5. doi: 10.1126/science.6101511.

Abstract

The endothelia of microvessels isolated from mouse brain by mechanical means are rich in gamma-glutamyl transpeptidase; however, the enzyme often disappears when the cells migrate or proliferate from the microvessel isolates. In an endothelial cell line derived from similar isolates and negative for gamma-glutamyl transpeptidase, the enzyme could be induced in the endothelial cells when they were cocultured with glial cells. Thus there may be a requirement for continuous induction of gamma-glutamyl transpeptidase in brain microvessels by adjacent glial cells.

摘要

通过机械方法从小鼠脑部分离出的微血管内皮富含γ-谷氨酰转肽酶；然而，当这些细胞从小微血管分离物中迁移或增殖时，该酶常常消失。在源自类似分离物且γ-谷氨酰转肽酶呈阴性的内皮细胞系中，当内皮细胞与神经胶质细胞共培养时，该酶能够在内皮细胞中被诱导产生。因此，脑微血管中的γ-谷氨酰转肽酶可能需要相邻神经胶质细胞的持续诱导。