Zhu X J, Yao A Q, Liu B
Institute of Cardiovascular Research, Beijing Medical University.
Zhonghua Yi Xue Za Zhi. 1994 Mar;74(3):158-60, 191.
pN2-LacZ or pN2-CMV-ProUK plasmid DNA was separately injected into rat quadricept muscles and beta-galactosidase or Pro-UK was detected after 2 days. The expression of foreign genes lasted for at least 3 months. pN2-LacZ plasmid was injected into the left ventricular wall of the rat, and the expression of LacZ gene was more efficient than in the quadricept muscle. pN2-LacZ was also introduced into the rat arterial wall by balloon catheter. A new method was also found to improve the efficiency of foreign gene expression in muscle by stitching medical stitch containing plasmid DNA onto the quadricept muscle.
将pN2-LacZ或pN2-CMV-ProUK质粒DNA分别注射到大鼠股四头肌中，2天后检测到β-半乳糖苷酶或Pro-UK。外源基因的表达持续了至少3个月。将pN2-LacZ质粒注射到大鼠左心室壁，LacZ基因的表达比在股四头肌中更有效。还通过球囊导管将pN2-LacZ导入大鼠动脉壁。还发现了一种新方法，即通过将含有质粒DNA的医用缝线缝到股四头肌上来提高外源基因在肌肉中的表达效率。
