Yao A Q, Sun S D, Zhu X J
Institute of Cardiovascular Research, Beijing Medical University.
Zhonghua Yi Xue Za Zhi. 1994 Apr;74(4):224-7, 255.
Three retroviral vectors containing report gene LacZ or the whole length cDNA of human Pro-UK and tPA, pN2-LacZ, pN2-CMV-ProUK and pN2-CMV-tPA were constructed. The rat vascular smooth muscle cells (VSMCs) were transfected with the plasmids by calcium phosphate coprecipitation or pseudovirus infection. The transfected cells were selected by G418. Southern blot analysis showed that the foreign genes were present in the genome of the transfected VSMC, and the expression products of these genes could be detected in the transfected VSMCs. These results suggested that the VSMC could be a targeting cell for gene therapy on cardiovascular diseases.
构建了三种含有报告基因LacZ或人Pro-UK及tPA全长cDNA的逆转录病毒载体，即pN2-LacZ、pN2-CMV-ProUK和pN2-CMV-tPA。采用磷酸钙共沉淀法或假病毒感染法将质粒转染大鼠血管平滑肌细胞（VSMC）。通过G418筛选转染细胞。Southern印迹分析表明，外源基因存在于转染VSMC的基因组中，且这些基因的表达产物可在转染的VSMC中检测到。这些结果提示，VSMC可能是心血管疾病基因治疗的靶向细胞。
