Gorunova L, Mertens F, Mandahl N, Jonsson N, Persson B, Heim S, Mitelman F
Department of Clinical Genetics, University Hospital, Lund, Sweden.
Cancer Genet Cytogenet. 1994 Oct;77(1):26-32. doi: 10.1016/0165-4608(94)90144-9.
Short-term cultures from a clear cell hidradenoma, a benign skin tumor for which no chromosome data exist, were cytogenetically analyzed. A total of eight unrelated aberrant clones were identified. The karyotypic profiles of two separately processed parts of the sample--a tumor nodule and seemingly normal adjacent dermal tissue--were different. Characteristic for the tumor nodule was a single abnormal clonal population consisting of three subclones: 46,XY,der(2)inv(2)(p13q23)t(2;9)(p13;q22), der(9)t(2;9)(q23;q22),t(11;19)(q21;p13),t(12;19)(q24;p13)/46,idem, inv(1)(p32q44)/92,idemx2. The adjacent tissue contained, in addition to the clone found in the tumor nodule, a spectrum of unrelated clones, the largest of which also showed clonal evolution: 45-47,XY,t(3;6)(p25;p25),t(12;17)(q15;q12),-17,+r(17)x2 [cp]/45-47,idem,inv(5)(p15q22)/90-94,idemx2. The remaining six clones found in this part were small and had simpler numerical or structural aberrations. The multiclonal pattern observed in this hidradenoma seems to reflect both cytogenetic convergence and divergence during neoplastic progression. The presence of unrelated clones may be an indication that the tumor was of multicellular origin.
对透明细胞汗腺瘤（一种尚无染色体数据的良性皮肤肿瘤）进行了短期培养，并进行了细胞遗传学分析。共鉴定出8个不相关的异常克隆。样本的两个分别处理的部分——肿瘤结节和看似正常的相邻真皮组织——的核型图谱不同。肿瘤结节的特征是一个由三个亚克隆组成的单一异常克隆群体：46,XY,der(2)inv(2)(p13q23)t(2;9)(p13;q22)，der(9)t(2;9)(q23;q22)，t(11;19)(q21;p13)，t(12;19)(q24;p13)/46, 同型，inv(1)(p32q44)/92, 同型x2。除了在肿瘤结节中发现的克隆外，相邻组织还包含一系列不相关的克隆，其中最大的克隆也显示出克隆进化：45 - 47,XY,t(3;6)(p25;p25)，t(12;17)(q15;q12)，-17，+r(17)x2 [cp]/45 - 47, 同型，inv(5)(p15q22)/90 - 94, 同型x2。在这部分中发现的其余6个克隆较小，具有更简单的数目或结构异常。在这种透明细胞汗腺瘤中观察到的多克隆模式似乎反映了肿瘤进展过程中的细胞遗传学趋同和分歧。不相关克隆的存在可能表明该肿瘤起源于多细胞。
