Schraml P, Shipman R, Colombi M, Ludwig C U
Kantonsspital Basel, Zentrum für Lehre und Forschung, Switzerland.
Cancer Res. 1994 Oct 1;54(19):5236-40.
Using a magnet-assisted subtraction technique, 17 complementary DNA (cDNA) clones were isolated that were expressed in the normal lung but were decreased or lost in the corresponding tumor tissue of a nonsmall cell lung carcinoma patient. The lack of expression of six magnet-assisted subtraction technique cDNA clones in three additional non-small cell lung carcinoma cases indicates their possible relevance for non-small cell lung carcinoma. Two cDNA clones revealed homology to genes specifically expressed in lung, i.e., pulmonary surfactant-associated protein B and the receptor for advanced glycosylation end products of proteins. Three cDNA clones showed identity to cDNA sequences encoding calmodulin-like protein, glutamine synthetase, and cytoskeletal beta-actin. One cDNA clone is identical to a recently described human expressed sequence tag whose gene is still unknown.
利用磁珠辅助消减技术，分离出17个互补DNA（cDNA）克隆，这些克隆在正常肺组织中表达，但在一名非小细胞肺癌患者的相应肿瘤组织中表达降低或缺失。另外3例非小细胞肺癌病例中6个磁珠辅助消减技术cDNA克隆的表达缺失，表明它们可能与非小细胞肺癌相关。两个cDNA克隆与在肺中特异性表达的基因具有同源性，即肺表面活性物质相关蛋白B和蛋白质晚期糖基化终产物受体。三个cDNA克隆与编码钙调蛋白样蛋白、谷氨酰胺合成酶和细胞骨架β-肌动蛋白的cDNA序列相同。一个cDNA克隆与最近描述的人类表达序列标签相同，其基因仍未知。
