Zhu G, Tao H, Huang Z
Shanghai Institute of Biological Products.
Zhonghua Liu Xing Bing Xue Za Zhi. 1994 Apr;15(2):80-2.
A procedure of keeping Danish BCG strain 2 is reported. The strain is cultivated in Bile potato Medium and Suton Potato Medium alternatively with incubation periods of two weeks. Lyophilized vaccine produced by using seeds derived from this strain gives better immunogenicity and lower side reaction to new-born babies and has better thermostability in compare with vaccines derived from strains of Beijing, Brazil, CCCP, France of Hungary.