一种由应激和热休克触发的新型激酶级联反应，可刺激丝裂原活化蛋白激酶相关蛋白激酶-2以及小热休克蛋白的磷酸化。

A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins.

作者信息

Rouse J, Cohen P, Trigon S, Morange M, Alonso-Llamazares A, Zamanillo D, Hunt T, Nebreda A R

机构信息

Department of Biochemistry, University of Dundee, Scotland.

出版信息

Cell. 1994 Sep 23;78(6):1027-37. doi: 10.1016/0092-8674(94)90277-1.

DOI:10.1016/0092-8674(94)90277-1

PMID:7923353

Abstract

MAPK-activated protein kinase-2 (MAPKAP kinase-2) is activated in vitro by the p42 and p44 isoforms of MAPK (p42/p44MAPK). In several cell lines, however, MAPKAP kinase-2 is activated by sodium arsenite, heat shock, or osmotic stress and not by agonists that activate p42/p44MAPK. We have identified a MAPK-like enzyme that acts as a MAPKAP kinase-2 reactivating kinase (RK). RK is recognized by an antiserum raised against a Xenopus MAPK (Mpk2), which is most similar to HOG1 from S. cerevisiae. We also identified a RK kinase (RKK) on the basis of its ability to activate either RK or a GST-Mpk2 fusion protein. The RKK, RK, and MAPKAP kinase-2 constitute a new stress-activated signal transduction pathway in vertebrates that is distinct from the classical MAPK cascade.

摘要

丝裂原活化蛋白激酶激活的蛋白激酶2（MAPKAP激酶2）在体外被丝裂原活化蛋白激酶（p42/p44MAPK）的p42和p44亚型激活。然而，在几种细胞系中，MAPKAP激酶2被亚砷酸钠、热休克或渗透压应激激活，而不被激活p42/p44MAPK的激动剂激活。我们鉴定出一种类丝裂原活化蛋白激酶，它作为MAPKAP激酶2的再激活激酶（RK）起作用。RK可被针对非洲爪蟾丝裂原活化蛋白激酶（Mpk2）产生的抗血清识别，Mpk2与酿酒酵母的HOG1最为相似。我们还基于其激活RK或GST-Mpk2融合蛋白的能力鉴定出一种RK激酶（RKK）。RKK、RK和MAPKAP激酶2在脊椎动物中构成了一条新的应激激活信号转导途径，该途径不同于经典的丝裂原活化蛋白激酶级联反应。