Cunarro J A, Johnson W A, Uehling D T, Updike S J, Weiner M W
J Lab Clin Med. 1976 Dec;88(6):873-84.
The metabolic consequences of low-temperature kidney preservation were investigated. A comparison was made between kidneys which were immediately preserved and kidneys which had been ischemic for 1 hour. Two types of preservation techniques were used: (1) continuous perfusion with oxygenated plasma as described by Belzer and (2) a single flush with potassium-containing perfusate as suggested by Collins. Slices of renal cortex were removed at varying times during preservation and analyzed for a variety of metabolic intermediates. ATP levels were markedly reduced from normal. The Belzer technique was associated with higher ATP levels and ischemia lowered the ATP level. Kidneys perfused by the Belzer technique had lower ADP levels than those by the Collins method. Preservation caused marked elevation of tissue lactate, irrespective of ischemia or the technique used. We conclude that low temperature kidney preservation has profound effects on cellular metabolism. Therefore, the measurement of metabolic intermediates may provide a rational approach to the prediction of organ survival.
研究了低温肾脏保存的代谢后果。对立即保存的肾脏和缺血1小时的肾脏进行了比较。使用了两种保存技术：（1）如Belzer所述的用含氧血浆持续灌注，以及（2）如Collins所建议的用含钾灌注液单次冲洗。在保存过程中的不同时间取出肾皮质切片，并分析各种代谢中间产物。ATP水平较正常明显降低。Belzer技术与较高的ATP水平相关，而缺血会降低ATP水平。用Belzer技术灌注的肾脏比用Collins方法灌注的肾脏ADP水平更低。无论是否存在缺血或所使用的技术，保存都会导致组织乳酸显著升高。我们得出结论，低温肾脏保存对细胞代谢有深远影响。因此，代谢中间产物的测量可能为预测器官存活提供一种合理的方法。
