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[猫丘脑后核群(PO)内脏伤害性神经元细胞内电位记录及其细胞内HRP标记]

[Recording intracellular potentials of viscero-nociceptive neurons in posterior group of thalamic nuclei (PO) and their labeling with HRP intracellularly in cats].

作者信息

Teng G, Meng X, Liu S, Ma X

机构信息

Laboratory of Neurophysiology, China Medical Unviersity, Shenyang.

出版信息

Zhen Ci Yan Jiu. 1993;18(4):262-6.

PMID:7923730
Abstract

Intracellular potentials (ICP) of the neurons in PO evoked by stimulating greater splanchnic nerve (GSN) were recorded in 42 cats. The resting potentials and action potentials induced by stimulating GSN were 53.74 +/- 11.68 mV and 29.44 +/- 9.22 mV respectively. Owing to intensity of the stimulation we can record EPSP and single spike or polyspikes on it. The action potentials derived from stimulating GSN (related action potentials) were inhibited by injection of morphine intravenously. On the basis of recording ICP, we also labeled 2 related neurons with stimulation of GSN and 2 unrelated ones. Differences between two kinds of the neurons were compared preliminarily. The ICP of the related neuron with stimulating GSN were inhibited by stimulating Amygdala too. Factors influencing amplitude of action potential in intracellular recording, criteria of determining nociceptive neuron and inhibition derived from stimulating Amygdala were also discussed.

摘要

在42只猫中记录了刺激内脏大神经(GSN)诱发的下丘脑神经元的细胞内电位(ICP)。刺激GSN诱发的静息电位和动作电位分别为53.74±11.68mV和29.44±9.22mV。由于刺激强度,我们可以在其上记录兴奋性突触后电位(EPSP)和单个峰电位或多峰电位。静脉注射吗啡可抑制刺激GSN产生的动作电位(相关动作电位)。在记录ICP的基础上,我们还用刺激GSN标记了2个相关神经元和2个不相关神经元。初步比较了两种神经元之间的差异。刺激杏仁核也可抑制刺激GSN时相关神经元的ICP。还讨论了细胞内记录中影响动作电位幅度的因素、伤害性神经元的判定标准以及刺激杏仁核产生的抑制作用。

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