Greenberg C S, Hursting M J, Macik B G, Ortel T L, Kane W H, Moore B M
Division of Hematology/Oncology, Duke University Medical Center, Durham, NC 27710.
Clin Chem. 1994 Oct;40(10):1962-9.
We have used a monoclonal antibody-based ELISA for plasma prothrombin fragment 1.2 (F1.2) to establish appropriate sample collection and storage conditions for this biomarker of thrombin generation. F1.2 concentrations were not altered by exogenous factor Xa, thrombin, or thromboplastin if blood was collected by routine venipuncture into tubes containing heparin as anticoagulant (but not citrate, acid-citrate-dextrose, EDTA, or oxalate) and if plasma antithrombin III concentration was > or = 30% of normal. Heparinized plasma F1.2 was stable for > or = 8 h at 20-25 degrees C, and if premixed with a stabilizing reagent, for > or = 4 years at -70 degrees C. Mean values for heparinized plasma F1.2 collected and stored by recommended procedures were increased in patients with thrombosis and conditions of increased thrombotic risk, and were sensitive to heparin and oral anticoagulant therapies. We conclude that plasma obtained by routine venipuncture into tubes with heparin as anticoagulant is an appropriate specimen for F1.2 measurements for most patients.
我们使用了一种基于单克隆抗体的酶联免疫吸附测定法来检测血浆凝血酶原片段1.2（F1.2），以确定这种凝血酶生成生物标志物的合适样本采集和储存条件。如果通过常规静脉穿刺将血液采集到含有肝素作为抗凝剂（而非柠檬酸盐、枸橼酸-葡萄糖、乙二胺四乙酸或草酸盐）的试管中，并且血浆抗凝血酶III浓度≥正常水平的30%，则F1.2浓度不会因外源性因子Xa、凝血酶或凝血活酶而改变。肝素化血浆中的F1.2在20 - 25℃下稳定≥8小时，如果与一种稳定试剂预混合，则在-70℃下稳定≥4年。按照推荐程序采集和储存的肝素化血浆F1.2的平均值在血栓形成患者和血栓形成风险增加的情况下会升高，并且对肝素和口服抗凝治疗敏感。我们得出结论，对于大多数患者而言，通过常规静脉穿刺将血液采集到含有肝素作为抗凝剂的试管中所获得的血浆是用于F1.2测量的合适样本。
