Leroy-Matheron C, Gouault-Heilmann M
Haemostasis Laboratory, Henri Mondor University Hospital, Créteil, France.
Thromb Res. 1994 May 15;74(4):399-407. doi: 10.1016/0049-3848(94)90155-4.
In this study, we evaluated the effects of anticoagulants used in blood sampling on the measurements of coagulation activation markers F1 + 2, TAT, D-Dimers by Elisa methods. The study was carried out on normal subjects and patients with inherited deficiency of coagulation inhibitors, antithrombin III (ATIII) protein C (PC) and protein S (PS). Three different anticoagulant solutions were compared: 1) ACD/EDTA/adenosine/heparin, 2) EDTA/aprotinin/a synthetic thrombin inhibitor and 3) sodium citrate. The results showed that sodium citrate, commonly used in coagulation laboratories, is a suitable anticoagulant for the study of coagulation activation markers. In addition, the type of tubes (plastic tubes vs glass Vacutainer R tubes) used for blood sampling as well as the order of sampling (early or late after the phlebotomy procedure) did not influence the results. We concluded that assays of coagulation activation markers F1 + 2 and D-Dimers can be performed in samples collected routinely by haemostasis laboratory staff using Vacutainer R tubes with sodium citrate. Further investigations are needed to understand why TAT measurements gave a pattern of results quite different from F1 + 2 or D-Di measurements.
在本研究中，我们通过酶联免疫吸附测定（ELISA）方法评估了血液采样中使用的抗凝剂对凝血激活标志物F1 + 2、凝血酶 - 抗凝血酶复合物（TAT）、D - 二聚体测量结果的影响。该研究在正常受试者以及遗传性凝血抑制剂（抗凝血酶III（ATIII）、蛋白C（PC）和蛋白S（PS））缺乏的患者中进行。比较了三种不同的抗凝剂溶液：1）酸性枸橼酸盐葡萄糖（ACD）/乙二胺四乙酸（EDTA）/腺苷/肝素，2）EDTA/抑肽酶/一种合成凝血酶抑制剂，3）枸橼酸钠。结果表明，凝血实验室常用的枸橼酸钠是研究凝血激活标志物的合适抗凝剂。此外，用于血液采样的试管类型（塑料管与玻璃真空采血管）以及采样顺序（静脉穿刺操作后的早期或晚期）均不影响结果。我们得出结论，凝血激活标志物F1 + 2和D - 二聚体的检测可在止血实验室工作人员使用含枸橼酸钠的真空采血管常规采集的样本中进行。需要进一步研究以了解为何TAT测量结果与F1 + 2或D - 二聚体测量结果呈现出截然不同的模式。
