与大肠杆菌牛源菌株K99抗原相关的血凝和黏附特性。

Haemagglutinating and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli.

作者信息

Burrows M R, Sellwood R, Gibbons R A

出版信息

J Gen Microbiol. 1976 Oct;96(2):269-75. doi: 10.1099/00221287-96-2-269.

Abstract

The K99 antigen common to some bovine strains of Escherichia coli caused mannose-resistant haemagglutination of sheep erythrocytes and was shown to be responsible for the attachment of K99-positive bacteria to calf brush-border preparations because (i) strains grown at 18 degrees C did not produce K99 antigen, cause haemagglutination, or attach to brush borders; (ii) a K12 (K99+) recombinant strain showed both haemagglutinating activity and attachment to brush borders whereas, before it received the K99 plasmid, the recipient strain was negative in both respects; and (iii) cell-free extracts of K99 antigen showed haemagglutinating activity and inhibited the attachment of K99-positive organisms to brush borders. K99 antigen appears to be a virulence determinant in the pathogenesis of neonatal calf diarrhoea. It is readily demonstrated by haemagglutination and brush-border attachment tests.

摘要

某些大肠杆菌牛源菌株共有的K99抗原可引起绵羊红细胞的甘露糖抗性血凝反应，并且已证明它是K99阳性细菌附着于犊牛刷状缘制剂的原因，因为：（i）在18℃下生长的菌株不产生K99抗原，不引起血凝反应，也不附着于刷状缘；（ii）一株K12（K99 +）重组菌株既表现出血凝活性，又能附着于刷状缘，而在接受K99质粒之前，受体菌株在这两方面均为阴性；（iii）K99抗原的无细胞提取物表现出血凝活性，并抑制K99阳性生物体附着于刷状缘。K99抗原似乎是新生犊牛腹泻发病机制中的一种毒力决定因素。通过血凝试验和刷状缘附着试验很容易证明这一点。