Morris J A, Wray C, Sojka W J
J Med Microbiol. 1980 May;13(2):265-71. doi: 10.1099/00222615-13-2-265.
Lambs from suckling ewes vaccinated with the K99 antigen were resistant to challenge with K99-positive enteropathogenic Escherichia coli. Serum and colostrum from these ewes were compared with samples from control ewes to establish methods for monitoring vaccination and to determine the mechanism of protection. Vaccination stimulated production of K99 antibodies. These could be detected by an indirect haemagglutination test and a haemagglutination-inhibition test. Antiglobulin and gel-diffusion tests were less reliable. Experiments with brush-border cells from calf intestine showed that the antibodies were associated with anti-adhesive activity. The antibodies were predominantly IgG and did not neutralise the activity of heat-stable enterotoxin. It was concluded that neutralisation of the adhesive properties of the K99-positive E. coli by colostral antibodies significantly contributed to the resistance of the lambs from vaccinated ewes.
用K99抗原对哺乳母羊进行疫苗接种后，其所产羔羊对K99阳性肠致病性大肠杆菌的攻击具有抵抗力。将这些母羊的血清和初乳与对照母羊的样本进行比较，以建立监测疫苗接种的方法并确定保护机制。疫苗接种刺激了K99抗体的产生。这些抗体可以通过间接血凝试验和血凝抑制试验检测到。抗球蛋白试验和凝胶扩散试验的可靠性较低。用小牛肠道刷状缘细胞进行的实验表明，这些抗体与抗黏附活性有关。这些抗体主要是IgG，不会中和热稳定肠毒素的活性。得出的结论是，初乳抗体对K99阳性大肠杆菌黏附特性的中和作用显著有助于接种疫苗母羊所产羔羊的抵抗力。
