吖啶酯标记的DNA探针在Bactec 13A血培养中用于鉴定分枝杆菌的应用。
Use of acridinium-ester-labeled DNA probes for identification of mycobacteria in Bactec 13A blood cultures.
作者信息
Ephraim D A, Spitzer E D
机构信息
Department of Laboratories, University Hospital, Stony Brook, New York.
出版信息
Diagn Microbiol Infect Dis. 1994 Mar;18(3):137-9. doi: 10.1016/0732-8893(94)90081-7.
AccuProbe tests for mycobacteria (Gen-Probe) cannot be performed directly on Bactec 13A cultures because of interfering substances. This problem can be circumvented by subculturing to Bactec 12B media. Artifactual chemiluminescence greater than the positive cutoff was seen in 15 of 19 13A cultures containing Mycobacterium avium complex compared with 0 of 19 in 12B subcultures. Of the subcultures, 89% were tested after overnight incubation.
由于存在干扰物质，不能直接在Bactec 13A培养物上进行分枝杆菌AccuProbe检测（基因探针）。这个问题可以通过转种到Bactec 12B培养基来解决。在19份含有鸟分枝杆菌复合群的13A培养物中，有15份出现了高于阳性临界值的人为化学发光现象，而在12B转种培养物中，19份中为0份。在这些转种培养物中，89%是在过夜培养后进行检测的。
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