Scott D R, Besancon M, Sachs G, Helander H
Laboratory of Membrane Biology, Veterans Administration Medical Center West Los Angeles, Wadsworth Division, California 90073.
Dig Dis Sci. 1994 Oct;39(10):2118-26. doi: 10.1007/BF02090359.
The effect of inhibition of acid secretion on parietal cell morphology and the concentration of H,K-ATPase alpha-subunit protein was determined by electron microscopy and western blotting. Omeprazole or famotidine alone or in combination were used. Control animals showed a morphological stimulation index (0 = resting, 1.0 = fully stimulated) of 0.60; omeprazole treatment (1 mg/kg, twice a day) resulted a stimulation index of 0.63, famotidine injection (20 mg/kg twice a day) an index of 0.11, famotidine infusion (0.2 mg/hr) for five days an index of 0.38, and the combination of omeprazole and famotidine injection twice a day gave an index of 0.02. No change in the frequency of degenerating or damaged parietal cells was observed in any of the groups. In control animals, the number of lysosomes was 0.9/cell, with famotidine 1.8 and with omeprazole 5.6/cell. H/K-ATPase levels fell by about 25% with omeprazole and rose by about 23% with famotidine.
通过电子显微镜和蛋白质印迹法测定了胃酸分泌抑制对壁细胞形态和H，K - ATP酶α亚基蛋白浓度的影响。单独或联合使用奥美拉唑或法莫替丁。对照动物的形态刺激指数（0 = 静息，1.0 = 完全刺激）为0.60；奥美拉唑治疗（1mg/kg，每日两次）导致刺激指数为0.63，法莫替丁注射（20mg/kg，每日两次）指数为0.11，法莫替丁输注（0.2mg/hr）五天指数为0.38，奥美拉唑和法莫替丁联合注射每日两次指数为0.02。在任何组中均未观察到退化或受损壁细胞频率的变化。对照动物中，溶酶体数量为0.9/细胞，法莫替丁组为1.8/细胞，奥美拉唑组为5.6/细胞。奥美拉唑使H/K - ATP酶水平下降约25%，法莫替丁使其升高约23%。
