Kennedy M K, Picha K S, Shanebeck K D, Anderson D M, Grabstein K H
Department of Immunobiology, Immunex Research and Development Corporation, Seattle, WA 98101-2936.
Eur J Immunol. 1994 Oct;24(10):2271-8. doi: 10.1002/eji.1830241002.
Our results indicate that interleukin (IL)-12 is an important costimulator of antigen-dependent proliferation of murine Th1 clones. In addition, we demonstrate that IL-10 inhibits splenic antigen-presenting cell (APC)-dependent proliferation of Th1 clones, at least in part, via down-regulation of APC-derived IL-12. Moreover, the failure of activated B cells to provide costimulation via IL-12 accounts for their inability to support optimal proliferative responses of Th1 clones. We also show that IL-12 regulates the ability of Th1 clones to respond to IL-4 and enhances their proliferation in response to IL-2, IL-7, or IL-15. In contrast, Th2 and Th0 clones appear refractory to the effects of IL-12, on antigen-dependent or growth factor-induced proliferation.
我们的结果表明，白细胞介素（IL）-12是小鼠Th1克隆抗原依赖性增殖的重要共刺激因子。此外，我们证明IL-10至少部分地通过下调抗原呈递细胞（APC）衍生的IL-12来抑制Th1克隆的脾脏APC依赖性增殖。此外，活化的B细胞无法通过IL-12提供共刺激，这解释了它们无法支持Th1克隆的最佳增殖反应。我们还表明，IL-12调节Th1克隆对IL-4的反应能力，并增强它们对IL-2、IL-7或IL-15的增殖反应。相比之下，Th2和Th0克隆对抗原依赖性或生长因子诱导的增殖似乎对IL-12的作用不敏感。
