Dartt D A, Rose P E, Dicker D M, Ronco L V, Hodges R R
Schepens Eye Research Institute, Boston, MA 02114.
Exp Eye Res. 1994 Apr;58(4):423-9. doi: 10.1006/exer.1994.1035.
The alpha-adrenergic stimulation by phenylephrine of lacrimal gland protein secretion was pharmacologically characterized. Acini, prepared from rat exorbital lacrimal glands, were incubated with agonists, antagonists or both for 0-20 min. Peroxidase secretion, an index of protein secretion, was measured spectrophotometrically. Peroxidase secretion was stimulated by the alpha 1-adrenergic agonists clonidine. The non-selective alpha-adrenergic antagonist phentolamine completely inhibited phenylephrine-induced secretion. The selective alpha 1-adrenergic alkylating agent phenoxybenzamine and the selective alpha 1-adrenergic antagonist prazosin partially inhibited phenylephrine-induced secretion. The alpha 2-adrenergic antagonist yohimbine, the beta-adrenergic antagonist timolol, and the dopaminergic antagonist haloperidol also inhibited phenylephrine-induced secretion but were 100-fold less potent than prazosin. It is concluded that phenylephrine activates an alpha 1-adrenergic pathway, but not an alpha 2-adrenergic, beta-adrenergic or dopaminergic pathway, to stimulate lacrimal gland protein secretion from acini.
对去氧肾上腺素通过α-肾上腺素能刺激泪腺蛋白质分泌进行了药理学表征。从大鼠眶外泪腺制备的腺泡与激动剂、拮抗剂或两者一起孵育0 - 20分钟。通过分光光度法测量作为蛋白质分泌指标的过氧化物酶分泌。过氧化物酶分泌受到α1-肾上腺素能激动剂可乐定的刺激。非选择性α-肾上腺素能拮抗剂酚妥拉明完全抑制去氧肾上腺素诱导的分泌。选择性α1-肾上腺素能烷基化剂酚苄明和选择性α1-肾上腺素能拮抗剂哌唑嗪部分抑制去氧肾上腺素诱导的分泌。α2-肾上腺素能拮抗剂育亨宾、β-肾上腺素能拮抗剂噻吗洛尔和多巴胺能拮抗剂氟哌啶醇也抑制去氧肾上腺素诱导的分泌，但效力比哌唑嗪低100倍。结论是，去氧肾上腺素激活α1-肾上腺素能途径而非α2-肾上腺素能、β-肾上腺素能或多巴胺能途径来刺激腺泡的泪腺蛋白质分泌。
