人胰腺癌中DU-PAN-2的神经节苷脂抗原

Ganglioside antigen of DU-PAN-2 in a human pancreatic cancer.

作者信息

Hamanaka Y, Hamanaka S, Shinagawa Y, Suzuki T, Inagaki F, Suzuki M, Suzuki A

机构信息

Second Department of Surgery, Yamaguchi University School of Medicine, Japan.

出版信息

FEBS Lett. 1994 Oct 10;353(1):48-52. doi: 10.1016/0014-5793(94)01004-8.

DOI:10.1016/0014-5793(94)01004-8

PMID:7926020

Abstract

DU-PAN-2 reactive gangliosides were isolated from the tumor of a patient with pancreatic cancer (duct cell carcinoma, moderately differentiated adenocarcinoma), having a negative Lewis blood phenotype, and were analyzed by means of TLC-immunostaining, enzyme-linked immunosorbent assay (ELISA), permethylation study, 1H NMR spectroscopy and fast atom bombardment mass spectrometry. The structures of the gangliosides were found to be NeuAc alpha 2-3Gal beta 1-3GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1'Cer, containing normal and hydroxy fatty acids. By TLC-immunostaining and ELISA with chemically synthesized gangliosides, DU-PAN-2 was demonstrated to react strongly with IV3 alpha NeuAc-Lc4Cer, weakly with IV3 alpha NeuAc-nLc4Cer, and moderately with IV6 alpha NeuAc-Lc4Cer and IV6 alpha NeuAc-nLc4Cer. Thus it was concluded that the DU-PAN-2 reactive ganglioside in the tumor is IV3 alpha NeuAc-Lc4Cer and that DU-PAN-2 has a rather broad specificity.

摘要

从一名Lewis血型阴性的胰腺癌（导管细胞癌，中度分化腺癌）患者的肿瘤中分离出DU-PAN-2反应性神经节苷脂，并通过薄层层析免疫染色、酶联免疫吸附测定（ELISA）、全甲基化研究、1H核磁共振光谱和快原子轰击质谱进行分析。发现这些神经节苷脂的结构为NeuAcα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glcβ1-1'Cer，含有正常脂肪酸和羟基脂肪酸。通过薄层层析免疫染色以及与化学合成神经节苷脂的ELISA检测，结果表明DU-PAN-2与IV3αNeuAc-Lc4Cer反应强烈，与IV3αNeuAc-nLc4Cer反应较弱，与IV6αNeuAc-Lc4Cer和IV6αNeuAc-nLc4Cer反应中等。因此得出结论，肿瘤中的DU-PAN-2反应性神经节苷脂为IV3αNeuAc-Lc4Cer，并且DU-PAN-2具有相当广泛的特异性。