在人类胎粪中检测到一种新的乳糖四糖系列神经节苷脂，GalNAc-3'-isoLM1。
A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium.
作者信息
Fredman P, Månsson J E, Wikstrand C J, Vrionis F D, Rynmark B M, Bigner D D, Svennerholm L
机构信息
Department of Psychiatry and Neurochemistry, Gothenburg University, St. Jörgen Hospital, Hisings Backa, Sweden.
出版信息
J Biol Chem. 1989 Jul 25;264(21):12122-5.
A monoclonal antibody produced by immunization with cells of the human glioma cell line D-54 MG reacted with ganglioside GM2. The binding epitope of the antibody was found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal. Immunological detection of glycolipid antigens on thin-layer plates with this monoclonal antibody, DMAb-1, revealed the presence of a new ganglioside. This ganglioside, co-migrating with NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer(6'-LM1) and GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GalNAc beta 1-4Gla beta 1-4Glc beta 1-1Cer (GalNAc-isoGM1) at chromatographic separation was isolated from human meconium. Its structure was determined by permethylation and fast atom bombardment-mass spectometry analyses. The new ganglioside was found to be a combination of the lacto and ganglio series gangliosides, and the structure found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GlcNAc alpha 1-3Gal beta 1-4Glc beta 1-1Cer(GalNAc-3'-isoLM1).
用人胶质瘤细胞系D - 54 MG的细胞免疫产生的单克隆抗体与神经节苷脂GM2发生反应。发现该抗体的结合表位为GalNAcβ1 - 4(NeuAcα2 - 3)Gal。用这种单克隆抗体DMAb - 1在薄层板上对糖脂抗原进行免疫检测，揭示了一种新神经节苷脂的存在。这种神经节苷脂在色谱分离时与NeuAcα2 - 6Galβ1 - 4GlcNAcβ1 - 3Galβ1 - 4Glcβ1 - 1Cer(6'-LM1)和GalNAcβ1 - 4(NeuAcα2 - 3)Galβ1 - 3GalNAcβ1 - 4Glaβ1 - 4Glcβ1 - 1Cer(GalNAc - isoGM1)共同迁移，从人胎粪中分离得到。通过全甲基化和快原子轰击质谱分析确定了其结构。发现这种新神经节苷脂是乳糖系列和神经节系列神经节苷脂的组合，其结构为GalNAcβ1 - 4(NeuAcα2 - 3)Galβ1 - 3GlcNAcα1 - 3Galβ1 - 4Glcβ1 - 1Cer(GalNAc - 3'-isoLM1)。
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