Kwiatowski J, Latorre A, Skarecky D, Ayala F J
Department of Ecology and Evolutionary Biology, University of California, Irvine 92717.
Gene. 1994 Sep 30;147(2):295-6. doi: 10.1016/0378-1119(94)90085-x.
A Cu/Zn superoxide dismutase-encoding gene (Sod) from Drosophila willistoni was cloned and sequenced. The gene shows a typical structure for a fruit-fly Sod gene, with a coding region of 462 bp in two exons separated by a 417-bp intron. Comparison of the Sod sequences from D. willistoni and D. melanogaster suggests that these species are only remotely related. Downstream from the Sod gene, there is an ORF on the opposite strand that putatively encodes the last exon of an unidentified gene. The polyadenylation signals of the two genes are separated by only 61 bp in D. willistoni, conforming to the common picture of compact dipteran genomes.
克隆并测序了来自威氏果蝇的一个编码铜/锌超氧化物歧化酶的基因(Sod)。该基因显示出果蝇Sod基因的典型结构,其编码区为462 bp,分布在两个外显子中,中间由一个417 bp的内含子隔开。威氏果蝇和黑腹果蝇的Sod序列比较表明,这两个物种的亲缘关系较远。在Sod基因下游,反义链上有一个开放阅读框,推测它编码一个未知基因的最后一个外显子。在威氏果蝇中,这两个基因的聚腺苷酸化信号仅相隔61 bp,符合双翅目基因组紧凑的常见情况。