Arhontaki Kyriaki, Eliopoulos Elias, Goulielmos George, Kastanis Petros, Tsakas Spyros, Loukas Michael, Ayala Francisco
Department of Genetics, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.
J Mol Evol. 2002 Dec;55(6):745-56. doi: 10.1007/s00239-002-2370-9.
The phylogenetic relationships among the Drosophila melanogaster subgroup species were analyzed using approximately 1550-nucleotide-long sequences of the Cu,Zn SOD gene. Phylogenetic analysis was performed using separately the whole region and the intron sequences of the gene. The resulting phylogenetic trees reveal virtually the same topology, separating the species into distinct clusters. The inferred topology generally agrees with previously proposed classifications based on morphological and molecular data. The amino acid sequences of the Cu,Zn SOD of the D. melanogaster subgroup species reveal a high-conservation pattern. Only 3.9% of the total amino acid sites are variable, and none affects the major structural elements. Comparison of the Drosophila Cu,Zn SOD amino acid sequences with the Cu,Zn SOD of Bos taurus and Xenopus laevis (whose three-dimensional structure has been elucidated) reveals conservation of all the protein's functionally important amino acids and no substitutions that dramatically change the charge or the polarity of the amino acids.
利用约1550个核苷酸长的铜锌超氧化物歧化酶（Cu,Zn SOD）基因序列，分析了黑腹果蝇亚组物种之间的系统发育关系。分别使用该基因的全区域序列和内含子序列进行系统发育分析。所得的系统发育树显示出几乎相同的拓扑结构，将这些物种分为不同的簇。推断出的拓扑结构总体上与先前基于形态学和分子数据提出的分类一致。黑腹果蝇亚组物种的铜锌超氧化物歧化酶氨基酸序列呈现出高度保守的模式。在总的氨基酸位点中，只有3.9%是可变的，并且没有一个影响主要结构元件。将果蝇的铜锌超氧化物歧化酶氨基酸序列与已阐明三维结构的牛和非洲爪蟾的铜锌超氧化物歧化酶进行比较，结果显示该蛋白质所有功能重要的氨基酸均保守，并且没有取代会显著改变氨基酸的电荷或极性。
