Tamori A, Nishiguchi S, Kuroki T, Seki S, Kobayashi K, Kinoshita H, Otani S
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Hepatology. 1994 Nov;20(5):1179-86.
To evaluate the correlation between the polyamine metabolism and the degree of malignancy in hepatocellular carcinoma, we measured ornithine decarboxylase activity and polyamine concentrations in neoplastic tissue and adjacent noncancerous tissue from resected specimens of liver from 30 patients. Ornithine decarboxylase activity, polyamines (putrescine, spermidine and spermine) and ornithine decarboxylase mRNA levels were significantly higher in hepatoma tissue than in noncancerous tissue. The activity of this enzyme in the tumor tissue had a negative correlation with the histological degree of differentiation judged according to a modification of the Edmondson and Steiner classification. Resected hepatoma tissue was stained immunohistochemically with antibodies for proliferating cell nuclear antigen (also called cyclin), a marker of cell proliferation. We noted correlation between ornithine decarboxylase activity and the number of cells stained for this antigen (r = 0.882, p < 0.001). These results indicate that ornithine decarboxylase activity is high in human hepatocellular carcinoma, leading to increased intracellular concentrations of polyamines. Ornithine decarboxylase activity also reflected the rate of tumor proliferation and was correlated with the histological findings.
为评估多胺代谢与肝细胞癌恶性程度之间的相关性，我们测定了30例肝切除标本中肿瘤组织及邻近非癌组织中的鸟氨酸脱羧酶活性和多胺浓度。肝癌组织中的鸟氨酸脱羧酶活性、多胺（腐胺、亚精胺和精胺）及鸟氨酸脱羧酶mRNA水平显著高于非癌组织。根据Edmondson和Steiner分类法的改良版判断，肿瘤组织中该酶的活性与组织学分化程度呈负相关。用增殖细胞核抗原（也称为细胞周期蛋白）抗体对切除的肝癌组织进行免疫组织化学染色，增殖细胞核抗原是细胞增殖的标志物。我们发现鸟氨酸脱羧酶活性与该抗原染色阳性的细胞数量之间存在相关性（r = 0.882，p < 0.001）。这些结果表明，人肝细胞癌中鸟氨酸脱羧酶活性较高，导致细胞内多胺浓度升高。鸟氨酸脱羧酶活性还反映了肿瘤增殖速率，并与组织学结果相关。
