Weiss Thomas S, Bernhardt Günther, Buschauer Armin, Thasler Wolfgang E, Dolgner Doris, Zirngibl Hubert, Jauch Karl-Walter
Department of Surgery, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93042 Regensburg, Germany.
Int J Colorectal Dis. 2002 Nov;17(6):381-7. doi: 10.1007/s00384-002-0394-7. Epub 2002 Apr 20.
Cellular proliferation and differentiation are regulated by polyamines and their rate-limiting enzyme ornithine decarboxylase (ODC), both of which are correlated with tumor growth, but their role in differentiation is less clear. We investigated the correlation of ODC activity and polyamine levels with tumor stage and grade with respect to sample recruitment.
We determined ODC activity ([(14)C]CO(2) release), polyamines (HPLC), and histological staging and grading (TNM classification) of tissue samples from 64 patients with colorectal adenocarcinomas.
We found the concentrations of putrescine, spermidine, and N(1)-acetyl-spermidine and the ODC activity in tumor tissue to be twice as high as in adjacent normal mucosa. A critical parameter affecting ODC activity was ischemic time, which significantly reduced ODC activity levels in tumors (threefold) and in the surrounding normal tissue (ninefold) when the ischemic period exceeded 1 h. By contrast, polyamine content was not affected by ischemia. Total polyamine and spermine concentrations were higher in T3 and T4 than in T2 tumors, but putrescine was higher in T4 than in T3 and T2 tumors. There were significantly higher levels of total polyamines and spermine in moderately differentiated (G2) than in poorly differentiated (G3) tumors.
The lower spermidine/spermine ratio in G2 (0.44) compared with that in G3 (0.64) tumors suggests the involvement of the polyamines in colonic cell differentiation. Polyamine content is thus correlated with the tumor stage.
细胞增殖和分化受多胺及其限速酶鸟氨酸脱羧酶（ODC）调控，二者均与肿瘤生长相关，但其在分化中的作用尚不清楚。我们就样本采集情况，研究了ODC活性和多胺水平与肿瘤分期及分级的相关性。
我们测定了64例结肠腺癌患者组织样本的ODC活性（[¹⁴C]CO₂释放量）、多胺（高效液相色谱法）以及组织学分期和分级（TNM分类）。
我们发现肿瘤组织中腐胺、亚精胺和N¹ - 乙酰亚精胺的浓度以及ODC活性是相邻正常黏膜的两倍。影响ODC活性的一个关键参数是缺血时间，当缺血时间超过1小时时，肿瘤组织（三倍）和周围正常组织（九倍）中的ODC活性水平显著降低。相比之下，多胺含量不受缺血影响。T3和T4期肿瘤的总多胺和精胺浓度高于T2期肿瘤，但T4期肿瘤中的腐胺高于T3和T2期肿瘤。中度分化（G2）肿瘤中的总多胺和精胺水平显著高于低分化（G3）肿瘤。
G2期肿瘤（0.44）中亚精胺/精胺比值低于G3期肿瘤（0.64），这表明多胺参与了结肠细胞分化。因此，多胺含量与肿瘤分期相关。
