Amano A, Sharma A, Sojar H T, Kuramitsu H K, Genco R J
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo 14214.
Infect Immun. 1994 Oct;62(10):4682-5. doi: 10.1128/iai.62.10.4682-4685.1994.
We examined the biosynthesis of fimbriae and superoxide dismutase (SOD) produced by the periodontopathic bacterium Porphyromonas gingivalis in response to elevated temperature. P. gingivalis 2561, grown at 37 degrees C to mid-logarithmic phase, was subsequently incubated at 39, 41, and 43 degrees C, respectively, to stationary phase. There was no difference in the growth of cells at 37 and 39 degrees C. However, at 39 degrees C there was a 54% reduction in the amount of fimbrillin (fimbriae) as well as decreased expression of mRNA for fimA. On the other hand, under the same conditions, a more than twofold increase in the amount of SOD activity, as well as in the levels of SOD mRNA, was observed. Moreover, cells cultured for 20 h at 39 degrees C showed an 86% decrease of fimbrillin protein and a threefold increase in SOD activity. These observations suggest that P. gingivalis may undergo alterations in its virulence and susceptibility to host immune responses as a result of the elevated temperatures found in inflamed periodontal pockets.
我们研究了牙周病原菌牙龈卟啉单胞菌在温度升高时菌毛和超氧化物歧化酶（SOD）的生物合成情况。将在37℃培养至对数中期的牙龈卟啉单胞菌2561分别在39℃、41℃和43℃培养至稳定期。在37℃和39℃时细胞生长没有差异。然而，在39℃时，菌毛蛋白（菌毛）的量减少了54%，同时fimA的mRNA表达也降低。另一方面，在相同条件下，观察到SOD活性以及SOD mRNA水平增加了两倍多。此外，在39℃培养20小时的细胞显示菌毛蛋白减少了86%，而SOD活性增加了三倍。这些观察结果表明，由于炎症牙周袋中温度升高，牙龈卟啉单胞菌的毒力及其对宿主免疫反应的敏感性可能会发生改变。
