Thompson C H, Rose B R, Elliott P M
Department of Infectious Diseases, Faculty of Medicine, University of Sydney, New South Wales, Australia.
Gynecol Oncol. 1994 Jul;54(1):40-6. doi: 10.1006/gyno.1994.1163.
To investigate the possibility of human cytomegalovirus (HCMV) involvement in the aetiology of cervical carcinoma or in the development of a more clinically aggressive cancer cell phenotype, biopsies of cervical cancer from 103 women undergoing primary therapy for invasive (Stage Ia to IV) cervical cancer were investigated using a polymerase chain reaction (PCR) designed to detect sequences from the IE mtrII region of HCMV. PCR assays were also used on the same specimens to identify the presence of common human papillomavirus (HPV) types associated with cervical cancer (HPV 16, 18, 31, 33, 52, and 58). Of the 103 cancers examined, only 4 contained detectable HCMV DNAs, a proportion lower than that found by another Australian group investigating the cervical carriage of HCMV in women with normal cervices. In contrast, 89 of these cancers were positive for HPV DNA sequences, with HPV 16 (65/103) and HPV 18 (17/103) being most commonly detected. Three of the 4 HCMV-positive tumors were also positive for HPV 16 DNA. An examination of the relevant histopathological and clinical data revealed no evidence to support a contention that cancers positive for HCMV are associated with any unusual histologic cell types or with aggressive clinical behavior.
为了研究人类巨细胞病毒（HCMV）是否参与宫颈癌的病因学或更具临床侵袭性的癌细胞表型的发展，我们使用一种旨在检测HCMV IE mtrII区域序列的聚合酶链反应（PCR），对103名接受浸润性（Ia期至IV期）宫颈癌初次治疗的女性的宫颈癌活检样本进行了研究。PCR检测也用于相同的样本，以鉴定与宫颈癌相关的常见人类乳头瘤病毒（HPV）类型（HPV 16、18、31、33、52和58）的存在。在检查的103例癌症中，只有4例含有可检测到的HCMV DNA，这一比例低于另一个澳大利亚研究组在检查宫颈正常女性的宫颈HCMV携带情况时所发现的比例。相比之下，这些癌症中有89例HPV DNA序列呈阳性，其中最常检测到的是HPV 16（65/103）和HPV 18（17/103）。4例HCMV阳性肿瘤中有3例HPV 16 DNA也呈阳性。对相关组织病理学和临床数据的检查显示，没有证据支持这样一种观点，即HCMV阳性的癌症与任何不寻常的组织学细胞类型或侵袭性临床行为有关。
